<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13787" schemaVersion="0.65" public="true">
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        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-09-29</depositionDate>
            <releaseDate>2026-07-28</releaseDate>
            <updateDate>2026-07-28</updateDate>
        </keyDates>
        <title>Cryo-EM micrographs of a near fully-activated intermediate A2AR-mini-Gαβγ complex</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9535-0369</authorORCID>
            <firstName>Yifan</firstName>
            <lastName>Cheng</lastName>
            <organization type="academic">Department of Biochemistry and Biophysics, University of California, San Francisco</organization>
            <street>600 16th Street</street>
            <townOrCity>San Francisco</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94143</postOrZipCode>
        </correspondingAuthor>
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            <firstName>Yifan</firstName>
            <lastName>Cheng</lastName>
            <organization type="academic">Department of Biochemistry and Biophysics, University of California, San Francisco</organization>
            <street>600 16th Street</street>
            <townOrCity>San Francisco</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94143</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-6358-1867">Bi M</author>
            <author authorORCID="0000-0002-9686-2072">Wang X</author>
            <author>Wang J</author>
            <author>Xu J</author>
            <author>Sun W</author>
            <author>Adediwura VA</author>
            <author authorORCID="0000-0003-3714-1395">Miao Y</author>
            <author authorORCID="0000-0001-9535-0369">Cheng Y</author>
            <author authorORCID="0000-0003-0818-2972">Ye L</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>5R35GM140847</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>1R01GM149659</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>1R21ES035378</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>5R01GM132572</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>S10OD020054</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">2.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-13787</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-47951</emdbEntry>
            <emdbEntry>EMD-47952</emdbEntry>
            <emdbEntry>EMD-47953</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-6358-1867" order="1">Bi M</author>
                    <author authorORCID="0000-0002-9686-2072" order="2">Wang X</author>
                    <author order="3">Wang J</author>
                    <author authorORCID="0000-0002-1600-583X" order="4">Xu J</author>
                    <author authorORCID="0000-0001-6957-2507" order="5">Sun W</author>
                    <author authorORCID="0009-0008-3337-2747" order="6">Adediwura VA</author>
                    <author authorORCID="0000-0003-3714-1395" order="7">Miao Y</author>
                    <author authorORCID="0000-0001-9535-0369" order="8">Cheng Y</author>
                    <author authorORCID="0000-0003-0818-2972" order="9">Ye L</author>
                    <title>Structure and function of a near fully-activated intermediate GPCR-Gαβγ complex</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country>United States</country>
                    <issue>1</issue>
                    <volume>16</volume>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-025-56434-4</externalReferences>
                    <externalReferences type="pubmed">39875358</externalReferences>
                    <details>The micrographs are raw micrographs in .tiff format</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Structure and function of a near fully- activated intermediate GPCR-Gαβγ complex</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>8875</numImagesOrTiltSeries>
        <framesPerImage>79</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>79</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.835</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.835</pixelHeight>
        </dimensions>
        <details>The raw micrographs include data from the same protein collected across four grids. The first three datasets were acquired on Quantifoil Au grids, and the fourth was acquired on an UltrAu grid. All four datasets were combined for the final reconstruction.</details>
        <segmentationList/>
        <micrographsFilePattern>data/mbi_*/*.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
