REL
2025-08-14
2026-07-20
2026-07-20
Cryo-EM structure of SARS-CoV-2 spike protein in complex with human neutralizing antibody WRAIR-2008
0000-0002-6772-3997
Paul
Thomas
Henry M Jackson Foundation
503 Robert Grant Ave
Silver Spring
MD
United States
20910
0000-0002-6808-7232
Michael
Gordon
Joyce
Henry M Jackson Foundation
503 Robert Grant Ave
Silver Spring
MD
United States
20910
Jensen JLJ
Thomas PVT
Joyce MGJ
Department of Defense (DOD, United States)
W81XWH-18-2-0040
United States
928.7
10.6019/EMPIAR-13782
EMDB
molecule
EMD-48284
Jensen JLJ
Thomas PVT
Joyce MGJ
First-generation N-terminal domain supersite public antibodies retain activity against Omicron-derived lineages and protect mice against Omicron BA.5 challenge
mBio
United States
English
10.1128/mbio.01036-25
Motion corrected, SARS-CoV-2 spike protein in complex with human neutralizing antibody WRAIR-2008
/data/micrographs
micrographs - single frame
MRC
MRC
10567
1
32 BIT FLOAT
5760
0.873
4092
0.873
Motion corrected, SARS-CoV-2 spike protein in complex with human neutralizing antibody WRAIR-2008 collected using SerialEM on a K3 camera in counting, non-CDS mode on a JEOL CryoARM 200 operated at 200kV (Cs=2.7) in EFTEM mode with a 20eV slit. The nominal dose per movie was ~60 e/Å^2 and motion-correction was performed using CryoSPARC v4.4.0.
Picked particle stack is not included, but alignment metadata for final global reconstruction (EMDB: 48284 PDB: 9mi3 (global)) is included in refined_particles.star. Instructions for CryoSPARC or RELION import and reextraction are provided in README.txt.
data/micrographs/*.mrc
data/refined_particles.star
Static Masks used for Focused Local Refinement
/data/static_masks
reconstructed volumes
MRC
MRC
1
1
32 BIT FLOAT
512
1.023
512
1.023
Static Masks used for Focused Local Refinement EMDB: 47928 PDB: 9ecz (focused refinement)