<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13756" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-07-02</depositionDate>
            <releaseDate>2026-07-13</releaseDate>
            <updateDate>2026-07-13</updateDate>
        </keyDates>
        <title>Cryo electron micrographs of 3-methylcrotonyl-CoA carboxylase and its variable α-Cap Assemblies in Legionella pneumophila</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-4027-4386</authorORCID>
            <firstName>Clarissa</firstName>
            <middleName>L.</middleName>
            <lastName>Durie</lastName>
            <organization type="academic">Department of Biochemistry, School of Medicine, University of Missouri</organization>
            <street>503 S. College Ave  117 Schweitzer Hall</street>
            <townOrCity>Columbia</townOrCity>
            <stateOrProvince>Missouri</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>65201</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-4027-4386</authorORCID>
            <firstName>Clarissa</firstName>
            <middleName>L.</middleName>
            <lastName>Durie</lastName>
            <organization type="academic">Department of Biochemistry, School of Medicine, University of Missouri</organization>
            <townOrCity>Columbia</townOrCity>
            <country>United States</country>
            <postOrZipCode>65201</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9635-1021</authorORCID>
            <firstName>Amit</firstName>
            <lastName>Meir</lastName>
            <organization type="academic">School of Infection &amp; Immunity, University of Glasgow</organization>
            <street>120 University Place</street>
            <townOrCity>Glasgow</townOrCity>
            <country>United Kingdom</country>
            <postOrZipCode>G12 8TA</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-4027-4386">Durie CL</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R35GM150663</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>R35GM122564</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Medical Research Council (MRC, United Kingdom)</fundingBody>
                <code>MC_UU_00034/1</code>
                <country>United Kingdom</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">988.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-13756</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>organism</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-56113</emdbEntry>
            <emdbEntry>EMD-56149</emdbEntry>
            <emdbEntry>EMD-56131</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0003-3320-4336" order="1">Somarathne RP</author>
                    <author authorORCID="0009-0009-3313-229X" order="2">Shrestha R</author>
                    <author authorORCID="0009-0008-5869-5307" order="3">Zehra M</author>
                    <author authorORCID="0009-0009-2455-6285" order="4">Brodeur C</author>
                    <author authorORCID="0000-0002-8180-9893" order="5">Shumate AD</author>
                    <author authorORCID="0000-0003-2096-8310" order="6">Bhella D</author>
                    <author authorORCID="0009-0005-3393-9764" order="7">Lai WC</author>
                    <author authorORCID="0000-0001-9635-1021" order="8">Meir A</author>
                    <author authorORCID="0000-0002-4027-4386" order="9">Durie CL</author>
                    <editor authorORCID="0000-0003-4315-1872" order="1">Sathe R</editor>
                    <title>Substrate-enhanced filamentation of 3-methylcrotonyl-CoA carboxylase in &lt;i&gt;Legionella pneumophila&lt;/i&gt;</title>
                    <journal>Communications Biology</journal>
                    <journalAbbreviation>Commun. Biol.</journalAbbreviation>
                    <country>United States</country>
                    <language>English</language>
                    <details>The micrographs are motion corrected and dose weighted.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Motion-corrected dose-weighted micrographs of carboxylase dataset S2</name>
        <directory>/data</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>31641</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.75</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.75</pixelHeight>
        </dimensions>
        <details>Motion-corrected, dose-weighted MRC micrographs from the LpDotB dataset without substrates. These are processed single-frame averaged micrographs after motion correction, not raw multi-frame movies. Each MRC file corresponds to one processed micrograph. The files are MRC mode 12, corresponding to RELION float16 / IEEE754 binary16 format. Image dimensions and pixel size were verified from representative MRC headers.</details>
        <segmentationList/>
        <micrographsFilePattern>data/FoilHole_23856122_Data_23852378_19_20251023_143100_Fractions_patch_aligned_doseweighted.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
