REL
2026-06-07
2026-07-24
2026-07-24
Cryo-EM raw data of the flexible TagR dimer from Pseudomonas aeruginosa
0000-0002-6451-8656
Roman
Peter
Jakob
Department Biozentrum, University of Basel, Switzerland
Spitalstrasse 41
Basel
Basel Stadt
Switzerland
4056
0000-0001-5414-2088
Marek
Basler
Departement Biozentrum, University of Basel
Spitalstrasse 41
Basel
Basel Stadt
Switzerland
4056
Kolesnikov M
Prakash P
Basler M
Swiss National Science Foundation
10000935
Switzerland
990.7
10.6019/EMPIAR-13668
Exception
molecule
Kolesnikov M
Prakash P
Basler M
Membrane damage induces TagQ/TagR complex-mediated H1-T6SS assembly in Pseudomonas aeruginosa
Micrographs were collected on a Titan Krios G1 electron microscope (FEI) operated at 300 kV, equipped with a GIF Quantum LS imaging filter (Gatan) and a K2 Summit detector (Gatan) operating in counting mode, using SerialEM. A total of 6,336 movies were acquired at a nominal magnification of 165,000×, corresponding to a pixel size of 0.82 Å, at defocus values ranging from 0.8–2.0 μm. Each movie was collected over a 9 s exposure, fractionated into 40 frames, with a total dose of approximately 51 e−/Å2. Images were acquired using beam-image shift to record 4 images per hole, except for initial test images.
Raw micrographs of TagQR collected on a Titan Krios G1 with a Gatan K2 Summit detector
/data
micrographs - multiframe
TIFF
TIFF
6336
40
1
40
32 BIT FLOAT
3710
3710.0
3838
3838.0
Micrographs were collected on a Titan Krios G1 electron microscope (FEI) operated at 300 kV, equipped with a GIF Quantum LS imaging filter (Gatan) and a K2 Summit detector (Gatan) operating in counting mode, using SerialEM. A total of 6,336 movies were acquired at a nominal magnification of 165,000×, corresponding to a pixel size of 0.82 Å, at defocus values ranging from 0.8–2.0 μm. Each movie was collected over a 9 s exposure, fractionated into 40 frames, with a total dose of approximately 51 e−/Å2. Images were acquired using beam-image shift to record 4 images per hole, except for initial test images.