REL 2026-05-12 2026-07-07 2026-07-07 0000-0002-9373-4737 Sebastian Klinge Rockefeller University 1230 York Ave New York NY United States 10065 0000-0002-9373-4737 Sebastian Klinge Rockefeller University 1230 York Ave New York NY United States 10065 Piwowarczyk R Klinge S National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) 5R01GM145950-03 United States Chan Zuckerberg Initiative 2023-332391 United States 32.7 10.6019/EMPIAR-13634 EMDB molecule EMD-75577 EMD-75578 EMD-75579 EMD-75580 Piwowarczyk R Klinge S bioRxiv 2026 10.64898/2026.05.22.727207 Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 3 /data/ds3 micrographs - multiframe TIFF TIFF 30768 50 1 50 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.7 to -1.7 µm, and a total dose of 50.0 e-/Å2 (50 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 4 /data/ds4 micrographs - multiframe TIFF TIFF 9708 40 1 40 UNSIGNED BYTE 11520 0.545 8184 0.545

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 81,000× with a pixel size of 0.545 Å/pixel, a defocus range of -0.6 to -1.7 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 5 /data/ds5 micrographs - multiframe TIFF TIFF 10799 40 1 40 UNSIGNED BYTE 11520 0.545 8184 0.545

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 81,000× with a pixel size of 0.545 Å/pixel, a defocus range of -0.6 to -1.7 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 6 /data/ds6 micrographs - multiframe TIFF TIFF 10632 40 1 40 UNSIGNED BYTE 11520 0.545 8184 0.545

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 81,000× with a pixel size of 0.545 Å/pixel, a defocus range of -0.6 to -1.7 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Polished particle stacks of Pwp1-RNP and Nop12-RNP co-transcriptional pre-60S intermediates /data/particles picked particles - multiframe - processed MRCS MRCS 2544054 1 SIGNED 16 BIT INTEGER 300 variable 300 variable

Particle sets used to reconstruct the pre-60S intermediates from all datasets. The particles were polished in cryoSPARC. For each reconstruction, the provided STAR file contains the patch-CTF estimated defocus and CTF corrections, as well as the final Euler angles, shifts, and micrograph coordinates.