Time-resolved cryo-EM particle stacks

REL 2026-03-14 2026-06-08 2026-06-08 Time-resolved cryo-EM particle stacks 0000-0003-1717-0914 Eric Raymond Greene Department of Chemistry and Biochemistry, San Francisco State University 1600 Holloway Ave San Francisco CA United States 94312 0000-0003-1717-0914 Eric Raymond Greene Department of Chemistry and Biochemistry, San Francisco State University 1600 Holloway Ave San Francisco CA United States 94312 0000-0002-0350-5827 Justin Kollman Department of Biochemistry, University of Washington 1959 NE Pacific Street Seattle WA United States 98195 0000-0002-5080-2859 James Fraser Department of Bioengineering and Therapeutic Sciences, University of California San Francisco 600 16th Street San Francisco CA United States 94143 Greene E Muniz R Yamamura H Hoff SE Bajaj P Lee DJ Thompson EM Arada A Lee GM Bonomi M Kollman JM Fraser JS National Institutes of Health (NIH) F32GM144982 United States National Institutes of Health (NIH) R35 GM145238 United States National Institutes of Health (NIH) R24GM154186 United States 8.9 10.6019/EMPIAR-13621 EMDB molecule EMD-70843 Greene ER Muniz RS Kollman JM Fraser JS Product-stabilized filamentation by human glutamine synthetase allosterically tunes metabolic activity eLife eLife United Kingdom 2026 English 10.7554/eLife.108336.1 40631248

The particle stacks provided were from motion-corrected, dose-weighted, CTF Fit, and extracted with a box size of 512 pixels in cryoSPARC (v3.1; Patch Motion Correction and Patch CTF Fit). Data were collected at a total dosage of 40 e⁻/Å² with a pixel size of 0.86 Å/pixel.

Template picked particles from the 57s turnover dataset of human glutamine synthetase /data/time_resolved_GS_particle_stack/J146 picked particles - multiframe - processed MRC MRC 3224670 75 SIGNED 16 BIT INTEGER 512 0.86 512 0.86

Particle positions are noted in the .star file. The data are picked/extracted particle positions from 5482 micrographs. These particles were template picked and have not be aligned in 2D or 3D.

data/time_resolved_GS_particle_stack/J146.star data/time_resolved_GS_particle_stack/J146/extract/*_aligned_DW_particles.mrc Template picked particles from the 239s turnover dataset of human glutamine synthetase /data/time_resolved_GS_particle_stack/J147 picked particles - multiframe - processed MRC MRC 3250902 75 SIGNED 16 BIT INTEGER 512 0.86 512 0.86

Particle positions are noted in the .star file. The data are picked/extracted particle positions from 5872 micrographs. These particles were template picked and have not be aligned in 2D or 3D.

data/time_resolved_GS_particle_stack/J147.star data/time_resolved_GS_particle_stack/J147/extract/*_aligned_DW_particles.mrc Template picked particles from the 99s turnover dataset of human glutamine synthetase /data/time_resolved_GS_particle_stack/J161 picked particles - multiframe - processed MRC MRC 1626169 75 SIGNED 16 BIT INTEGER 512 0.86 512 0.86

Particle positions are noted in the .star file. The data are picked/extracted particle positions from 2947 micrographs. These particles were template picked and have not be aligned in 2D or 3D.

data/time_resolved_GS_particle_stack/J161.star data/time_resolved_GS_particle_stack/J161/extract/*_aligned_DW_particles.mrc Template picked particles from the 122s turnover dataset of human glutamine synthetase /data/time_resolved_GS_particle_stack/J162 picked particles - multiframe - processed MRC MRC 571230 75 SIGNED 16 BIT INTEGER 512 0.86 512 0.86

Particle positions are noted in the .star file. The data are picked/extracted particle positions from 1029 micrographs. These particles were template picked and have not be aligned in 2D or 3D.

data/time_resolved_GS_particle_stack/J162.star data/time_resolved_GS_particle_stack/J162/extract/*_aligned_DW_particles.mrc Template picked particles from the 517s turnover dataset of human glutamine synthetase /data/time_resolved_GS_particle_stack/J163 picked particles - multiframe - processed MRC MRC 687806 75 SIGNED 16 BIT INTEGER 512 0.86 512 0.86

Particle positions are noted in the .star file. The data are picked/extracted particle positions from 1195 micrographs. These particles were template picked and have not be aligned in 2D or 3D.

data/time_resolved_GS_particle_stack/J163.star data/time_resolved_GS_particle_stack/J163/extract/*_aligned_DW_particles.mrc