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    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-02-20</depositionDate>
            <releaseDate>2026-05-31</releaseDate>
            <updateDate>2026-05-31</updateDate>
        </keyDates>
        <title>Raw data for single-particle cryo-EM analysis of P. aeruginosa PA3214 in complex with the C-terminal peptide of PA3213</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-6050-2219</authorORCID>
            <firstName>Nicolas</firstName>
            <lastName>Coudray</lastName>
            <organization type="academic">Johns Hopkins University</organization>
            <townOrCity>Baltimore</townOrCity>
            <stateOrProvince>Maryland</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>21218</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-2570-0404</authorORCID>
            <firstName>Damian</firstName>
            <middleName>C</middleName>
            <lastName>Ekiert</lastName>
            <organization type="academic">Johns Hopkins University, Department of Biology</organization>
            <street>3400 N Charles Street Mudd Hall 209</street>
            <townOrCity>Baltimore</townOrCity>
            <stateOrProvince>Maryland</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>21218</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-0624-6178</authorORCID>
            <firstName>Gira</firstName>
            <lastName>Bhabha</lastName>
            <organization type="academic">Johns Hopkins University, Department of Biology</organization>
            <street>3400 N Charles Street Mudd Hall 209</street>
            <townOrCity>Baltimore</townOrCity>
            <stateOrProvince>Maryland</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>21218</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-8291-7722">Giacometti SI</author>
            <author authorORCID="0000-0002-6050-2219">Coudray N</author>
            <author authorORCID="0000-0003-0624-6178">Bhabha G</author>
            <author authorORCID="0000-0002-2570-0404">Ekiert DC</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>R35GM128777</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Science Foundation (NSF, United States)</fundingBody>
                <code>2021318502</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>The Pew Charitable Trusts</fundingBody>
                <code>00033055</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>P30CA016087</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Simons Foundation</fundingBody>
                <code>SF349247</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">2.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-13573</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-77072</emdbEntry>
            <emdbEntry>EMD-77073</emdbEntry>
            <emdbEntry>EMD-77074</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0002-8291-7722" order="1">Giacometti SI</author>
                    <author authorORCID="0000-0002-6050-2219" order="2">Coudray N</author>
                    <author authorORCID="0000-0003-0621-1303" order="3">Redler RL</author>
                    <author authorORCID="0000-0003-0624-6178" order="4">Bhabha G</author>
                    <author authorORCID="0000-0002-2570-0404" order="5">Ekiert DC</author>
                    <title>Interactions of outer membrane lipoproteins  &lt;i&gt;P. aeruginosa&lt;/i&gt;  PA3214 and  &lt;i&gt;E. coli&lt;/i&gt;  PqiC with their MCE protein binding partners, PA3213 and PqiB</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                    <year>2026</year>
                    <externalReferences type="doi">10.64898/2026.05.09.724024</externalReferences>
                    <details>These are raw movies corresponding to a sample in which the PA3211-14 operon was over-expressed in E. coli and purified by affinity chromatography using a tag on PA3211.  Reconstructions only clearly show PA3213 and PA3214. It is unclear if PA3211 and PA3212 are present in stoichiometric amounts in the micrographs acquired.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Raw movies for the tilted dataset</name>
        <directory>/data/tilted/rawdata</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3925</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.083</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.083</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/tilted/rawdata/*tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Raw movies for the tilted dataset</name>
        <directory>/data/tilted/rawdata</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6057</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.083</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.083</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/untilted/rawdata/*tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
