REL
2026-05-14
2026-06-25
2026-07-14
In situ cryo-ET dataset of primary human T cells
0000-0002-7397-1321
Martin
Beck
Max Planck Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt am Main
Hessen
Germany
60438
0000-0002-7397-1321
Martin
Beck
Max Planck Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt am Main
Hessen
Germany
60438
Kreysing JP
Penzo C
Lusic M
Beck M
Max Planck Society
NA
Germany
Chan Zuckerberg Initiative
2021-234666
United States
German Research Foundation (DFG)
240245660
Germany
332.0
10.6019/EMPIAR-13566
EMDB
cell
EMD-58100
EMD-58101
Kreysing JP
Cruz-Leon S
Betz J
Penzo C
Majtner T
Schreiber M
Turonova B
Lusic M
Hummer G
Beck M
Molecular architecture of heterochromatin at the nuclear periphery of primary human cells
bioRxiv
2025
English
10.1101/2025.04.09.647790
The data was collected using a dose-symmetric scheme starting from +8 degree pre-tilt and covering a range from -52 to +68 degrees, with a tilt increment of 2 degrees.
Kreysing JP
Cruz-León S
Betz J
Penzo C
Majtner T
Schreiber M
Turoňová B
Lusic M
Hummer G
Beck M
Molecular architecture of heterochromatin at the nuclear periphery of primary human cells
Nature communications
Nat Commun
1
17
2026
English
10.1038/s41467-026-75087-5
42399220
Raw, unaligned, gain-corrected frames of primary human T cells
/data/raw_data/frames
tilt series
MRC
MRC
14
10
SIGNED 16 BIT INTEGER
4096
1.895
4096
1.895
The mdoc metadata files are also provided.
Tilt cleaned, dose-filtered tilt-series of primary human T cells
/data/input_stacks
tilt series
MRC
MRC
14
1
32 BIT FLOAT
4096
1.895
4096
1.895
These tilt stacks were used for tomogram reconstruction in IMOD and novaCTF. The relevant alignment files (.xf and .tlt) are provided in the alignment_files folder.
Reconstructed tomograms (4x binning) of primary human T cells
/data/bin4_rec
reconstructed volumes
MRC
MRC
14
1
SIGNED 16 BIT INTEGER
1024
7.58
1024
7.58
All tomograms were reconstructed in IMOD with 4x binning and a sirt-like filter. Tomogram 019 was additionally denoised with cryoCARE and IsoNet2.