REL 2026-05-07 2026-06-24 2026-06-24 0000-0002-1506-909X Nikolaus Grigorieff University of Massachusetts Chan Medical School 368 Plantation Street, AS4-2049 Worcester Massachusetts United States 01605 0000-0002-1506-909X Nikolaus Grigorieff University of Massachusetts Chan Medical School 368 Plantation Street, AS4-2049 Worcester Massachusetts United States 01605 0000-0002-7554-5967 Dmitri N Ermolenko Department of Biochemistry and Biophysics and Center for RNA Biology 601 Elmwood Ave Rochester New York United States 14642 0000-0003-1588-717X Andrei A Korostelev University of Massachusetts Chan Medical School 368 Plantation Street, AS5-1049 Worcester Massachusetts United States 01605 Brilot AF Korostelev AA Ermolenko DN Grigorieff N Natural Sciences and Engineering Research Council (NSERC, Canada) none Canada National Institutes of Health (NIH) GM-62580 United States National Institutes of Health (NIH) GM-92424 United States National Institutes of Health (NIH) GM-99719 United States 512.1 10.6019/EMPIAR-13553 EMDB molecule EMD-5796 EMD-5797 EMD-5798 EMD-5799 EMD-5800 Brilot AF Korostelev AA Ermolenko DN Grigorieff N Proc Natl Acad Sci USA PNAS United States 52 110 20994 20999 2013 English 10.1073/pnas.1311423110 24324137

Particle stack and Frealign parameters of EF-G-bound 70S ribosomes

Single-particle images of EF-G bound 70S ribosomes /data picked particles - single frame - processed MRCS MRC 1341961 1 32 BIT FLOAT 320 1.04 320 1.04

Particles were semiautomatically selected using e2boxer’s swarm tool, followed by manual curation of the dataset. Defocus parameters were determined using CTFFIND3. Boxing was performed using batchboxer, with particles stored in a single stack (particle_stack.mrc). frealign_parameters.par contains rough particle alignment parameters, obtained using FREALIGN, as well as other imaging-related parameters.

data/particle_stack.mrc data