REL
2026-04-17
2026-05-20
2026-05-20
Cryo-EM structures of alpha5beta1 integrin in complex with NeoNectin
0000-0002-3058-1550
Rachel
Werther
Fred Hutch Cancer Center
Seattle
United States
98109
0000-0003-1909-5751
Melody
Campbell
Fred Hutchinson Cancer Center
Seattle
WA
United States
98109
Werther R
Nguyen A
Alamo KAE
Wang X
Campbell MG
The Pew Charitable Trusts
N/A
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35 GM147414-01
United States
National Institutes of Health/National Cancer Institute (NIH/NCI)
P30 CA015704-40; RRID:SCR_022611
United States
Other private
Seattle Cancer Consortium Safeway Inc Pilot Grant
United States
382.1
10.6019/EMPIAR-13542
EMDB
molecule
EMD-45655
Wang X
Guillem-Marti J
Kumar S
Lee DS
Cabrerizo-Aguado D
Werther R
Alamo KAE
Zhao YT
Nguyen A
Kopyeva I
Huang B
Li J
Hao Y
Li X
Brizuela-Velasco A
Murray A
Gerben S
Roy A
DeForest CA
Springer T
Ruohola-Baker H
Cooper JA
Campbell MG
Manero JM
Ginebra MP
Baker D
De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties
Advanced materials (Deerfield Beach, Fla.)
Adv Mater
United States
34
37
2025
English
10.1002/adma.202500872
40489013
One hundred frame movies were recorded in super-resolution mode with a super-resolution pixel size of 0.561 Å/px, a nominal magnification of 36kx, a nominal defocus range of 1.2 to 2.0 µm under focus, and an approximate dose of 50 electrons per square Å
Unaligned multi-frame images of integrin alpha5 beta1 and minibinder
/data/movies
micrographs - multiframe
TIFF
TIFF
389
100
UNSIGNED BYTE
11520
0.561
8184
0.561
One hundred frame movies were recorded in super-resolution mode with a super-resolution pixel size of 0.561 Å/px, a nominal magnification of 36kx, a nominal defocus range of 1.2 - 2.0 um under focus and an approximate dose of 50 electrons per square Å
Aligned particle stack of alpha5 beta1 integrin bound to minibinder
/data/particle_stack
picked particles - single frame - processed
MRC
MRC
72604
1
UNSIGNED BYTE
352
1.122
352
1.122
Polished particles in .mrc format and accompanying metadata in .star file
Movie gain reference
/data/micrograph_gain_ref
micrographs - single frame
MRC
MRC
1
1
32 BIT FLOAT
11520
0.556
8184
0.556
Gain reference for movie collection in .mrc format