<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13502" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-04-16</depositionDate>
            <releaseDate>2026-06-28</releaseDate>
            <updateDate>2026-06-28</updateDate>
        </keyDates>
        <title>Cryo-electron tomography of cryo-FIB milled ∆ponB Escherichia coli division site</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-9123-1132</authorORCID>
            <firstName>Paula</firstName>
            <lastName>Navarro</lastName>
            <organization type="academic">Department of Fundamental Microbiology, University of Lausanne</organization>
            <street>UNIL Sorge</street>
            <townOrCity>Lausanne</townOrCity>
            <stateOrProvince>Vaud</stateOrProvince>
            <country>Switzerland</country>
            <postOrZipCode>1015</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-9123-1132</authorORCID>
            <firstName>Paula</firstName>
            <lastName>Navarro</lastName>
            <organization type="academic">Department of Fundamental Microbiology, University of Lausanne</organization>
            <street>UNIL Sorge, Biophore building</street>
            <townOrCity>Lausanne</townOrCity>
            <stateOrProvince>Vaud</stateOrProvince>
            <country>Switzerland</country>
            <postOrZipCode>1015</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-1245-0987</authorORCID>
            <firstName>Andrea</firstName>
            <lastName>Vettiger</lastName>
            <organization type="academic">University of Lausanne</organization>
            <street>UNIL Sorge, Biophore</street>
            <townOrCity>Lausanne</townOrCity>
            <stateOrProvince>Vaud</stateOrProvince>
            <country>Switzerland</country>
            <postOrZipCode>1015</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <firstName>Thomas</firstName>
            <middleName>G.</middleName>
            <lastName>Bernhardt</lastName>
            <organization type="academic">Harvard Medical School</organization>
            <street>4 Blackfan Circle</street>
            <townOrCity>Boston</townOrCity>
            <stateOrProvince>Massachusetts</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>02115</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-9123-1132">Navarro PP</author>
            <author>Vettiger A</author>
            <author>Hadju R</author>
            <author>Ananda VY</author>
            <author>Lopez-Tavares A</author>
            <author>Schmid EW</author>
            <author>Walter JC</author>
            <author>Loose M</author>
            <author>Chao LH</author>
            <author>Bernhardt TG</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>P500PB_203143</code>
                <country>Switzerland</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>P2BSP3_188112</code>
                <country>Switzerland</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>P400PB_199252</code>
                <country>Switzerland</country>
            </grantReference>
            <grantReference>
                <fundingBody>European Molecular Biology Organization (EMBO)</fundingBody>
                <code>ALTF_89-2019</code>
                <country>Belgium</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>R35GM142553</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>R01AI083365</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Howard Hughes Medical Institute (HHMI)</fundingBody>
                <code>----</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>320030-236243</code>
                <country>Switzerland</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>TMSGI3_218251</code>
                <country>Switzerland</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>320030-236069</code>
                <country>Switzerland</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>CRSK-3_237167</code>
                <country>Switzerland</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">54.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-13502</entryDOI>
        <experimentType>FIB-SEM</experimentType>
        <scale>cell</scale>
    </admin>
    <crossReferences>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0002-9123-1132" order="1">Navarro PP</author>
                    <author authorORCID="0000-0002-1245-0987" order="2">Vettiger A</author>
                    <author order="3">Hadju R</author>
                    <author authorORCID="0000-0001-5590-1170" order="4">Ananda VY</author>
                    <author order="5">Lopez-Tavares A</author>
                    <author authorORCID="0000-0002-4662-5298" order="6">Schmid EW</author>
                    <author authorORCID="0000-0002-4186-7570" order="7">Walter JC</author>
                    <author authorORCID="0000-0001-7309-9724" order="8">Loose M</author>
                    <author authorORCID="0000-0002-4849-4148" order="9">Chao LH</author>
                    <author order="10">Bernhardt TG</author>
                    <title>The aPBP-type cell wall synthase PBP1b plays a specialized role in fortifying the Escherichia coli division site against osmotic rupture</title>
                    <journal>Nature Microbiology</journal>
                    <journalAbbreviation>Nat. Micro.</journalAbbreviation>
                    <country></country>
                    <year>2026</year>
                    <details>Unaligned multi-frame movies of tilt-series of cryo-FIB milled dividing E. coli. Aligned stacks and binned tomograms.</details>
                </journalCitation>
            </universalCitation>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0002-9123-1132" order="1">Navarro PP</author>
                    <author authorORCID="0000-0002-1245-0987" order="2">Vettiger A</author>
                    <author order="3">Hadju R</author>
                    <author authorORCID="0000-0001-5590-1170" order="4">Ananda VY</author>
                    <author order="5">López-Tavares A</author>
                    <author authorORCID="0000-0002-4662-5298" order="6">Schmid EW</author>
                    <author authorORCID="0000-0002-4186-7570" order="7">Walter JC</author>
                    <author authorORCID="0000-0001-7309-9724" order="8">Loose M</author>
                    <author authorORCID="0000-0002-4849-4148" order="9">Chao LH</author>
                    <author order="10">Bernhardt TG</author>
                    <title>The aPBP-type cell wall synthase PBP1b plays a specialized role in fortifying the Escherichia coli division site against osmotic rupture</title>
                    <journal>BioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1101/2025.04.02.646830</externalReferences>
                    <externalReferences type="pubmed">40236067</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
        <relatedEmpiarEntries>
            <empiarEntry>EMPIAR-11090</empiarEntry>
        </relatedEmpiarEntries>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of tilt-series of cryo-FIB milled ∆ponB Escherichia coli. Aligned stacks and binned tomograms are included.</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>61</numImagesOrTiltSeries>
        <framesPerImage>4</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>4</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>1.3523</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>1.3523</pixelHeight>
        </dimensions>
        <details>Unaligned multi-frame movies of tilt-series of cryo-FIB milled ∆ponB Escherichia coli. Aligned stacks and binned tomograms are included. mdoc files are included.</details>
        <segmentationList/>
        <micrographsFilePattern>data</micrographsFilePattern>
        <pickedParticlesFilePattern>data</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data</pickedParticlesDirectory>
    </imageSet>
</entry>
