CryoEM movies of Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation determined using the SPT Labtech chameleon in the presence of 0x SurfACT.

REL 2026-03-27 2026-04-15 2026-04-15 CryoEM movies of Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation determined using the SPT Labtech chameleon in the presence of 0x SurfACT. 0000-0001-6653-6682 Mark A Herzik Department of Chemistry and Biochemistry 9500 Gilman Drive, Natural Sciences Building 4105 La Jolla California United States 92093 0000-0001-6653-6682 Mark A Herzik Department of Chemistry and Biochemistry 9500 Gilman Drive, Natural Sciences Building 4105 La Jolla California United States 92093 Enos SE Cook BD Rahmani H Narehood SM Li Y Kuschnerus IC Redford HT Dukakis P Ji D Bachochin MJ Grotjahn DA Herzik MA National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) 5R35GM138206 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) 1S10OD032471 United States Searle Scholars Program Searle Scholars Program United States Other private Cottrell Scholars United States Other private George W. and Carol A. Lattimer Faculty Research Fellowship United States 217.6 10.6019/EMPIAR-13433 EMDB molecule EMD-75848 EMD-75849 Enos SE Cook BD Rahmani H Narehood SM Li Y Kuschnerus IC Redford HT Dukakis P Ji D Bachochin MJ Grotjahn DA Herzik MA A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM 10.64898/2026.03.17.712260

CryoEM movies of Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation determined using the SPT Labtech chameleon in the presence of 0x SurfACT. Data were collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2 with a defocus range of −0.75 to −2.5 µm. Data were collected automatically using EPU with aberration free image shift.

Unaligned multi-frame micrographs of Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation determined using the SPT Labtech chameleon in the presence of 0x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.

Aligned micrographs of Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation determined using the SPT Labtech chameleon in the presence of 0x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were aligned in cryoSPARC.

Gain file required for motion correction of unaligned micrographs of Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation. /data/gain micrographs - single frame MRC MRC 1 1 32 BIT FLOAT 4096 0.735 4096 0.735

Gain file required for motion correction of unaligned micrographs of Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation determined using the SPT Labtech chameleon in the presence of 0x SurfACT.