REL
2026-03-17
2026-03-30
2026-03-30
C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP
0000-0001-5582-2851
Mariusz
Czarnocki-Cieciura
International Institute of Molecular and Cell Biology in Warsaw
4 Ks. Trojdena Street
Warsaw
Poland
02-106
0000-0001-8632-0977
Marcin
Nowotny
International Institute of Molecular and Cell Biology in Warsaw
4 Ks. Trojdena Street
Warsaw
Poland
02-109
Nirwal S
Czarnocki-Cieciura M
Zajko W
Skowronek K
Szczepanowski RH
Nowotny M
Polish National Science Centre
2017/ 26/A/NZ1/01098
Poland
Other government
1/SOL/2021/2
Poland
European Union (EU)
101059801 - RACE
Poland
European Union (EU)
IN-MOL-CELL Infrastructure
Poland
3.6
10.6019/EMPIAR-13401
EMDB
molecule
EMD-51677
EMD-51690
EMD-51691
Nirwal S
Czarnocki-Cieciura M
Zajko W
Skowronek K
Szczepanowski RH
Nowotny M
Structural snapshots of the mechanism of ATP-dependent DNA damage recognition by UvrA
Nature communications
Nat Commun
1
17
2025
English
10.1038/s41467-025-67075-y
41381534
Unaligned multi-frame movies of C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP (untilted movies)
/data/Movies/230413_untilted
micrographs - multiframe
TIFF
TIFF
3369
40
UNSIGNED BYTE
5760
0.84
4092
0.84
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 0 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 40.73 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.1, -1.8, -1.5, -1.2, -0.9, -0.6 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details
data/Movies
data/EMD-516*_particles/EMD-516*_particlestack.star
data/EMD-516*_particles
Unaligned multi-frame movies of C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP (tilted movies)
/data/Movies/230413_tilted
micrographs - multiframe
TIFF
TIFF
6326
40
UNSIGNED BYTE
5760
0.84
4092
0.84
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 30 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 40.73 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.0, -1.5, -1.0 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details
data/Movies
data/EMD-516*_particles/EMD-516*_particlestack.star
data/EMD-516*_particles
Aligned particles of C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP (consensus map)
/data/EMD-51677_particles
picked particles - single frame - processed
MRCS
MRCS
123699
1
32 BIT FLOAT
384
0.84
384
0.84
Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.
data/Movies
data/EMD-51677_particles/EMD-51677_particlestack.star
data/EMD-51677_particles
Aligned particles of C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP (focused map)
/data/EMD-51690_particles
picked particles - single frame - processed
MRCS
MRCS
136850
1
32 BIT FLOAT
256
0.84
256
0.84
Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.
data/Movies
data/EMD-51690_particles/EMD-51690_particlestack.star
data/EMD-51690_particles