REL 2026-02-27 2026-07-27 2026-07-27 0000-0002-7397-1321 Martin Beck Max Planck Institute of Biophysics Max-von-Laue-Strasse 3 Frankfurt am Main Hessen Germany 60438 0000-0002-7397-1321 Martin Beck Max Planck Institute of Biophysics, Department of Molecular Sociology Max-von-Laue-Strasse 3 Frankfurt am Main Hessen Germany 60438 Tuijtel MW Beck M Chan Zuckerberg Initiative 2021-234666 United States 707.5 10.6019/EMPIAR-13391 EMDB cell EMD-57021 Tuijtel MW Majtner M Turoňová B Beck M eLife English 10.7554/eLife.111639.1 Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 1-degree tilt increment /data/Data_1degree tilt series MRC MRC 63 1 SIGNED 16 BIT INTEGER 4096 1.971 4096 1.971

Tilt-series were aimed to collect 121 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided. Tilt images that were discarded during processing are specified in "tilt_clean_1deg.txt". Alignment results from IMOD are provided as TS_XXXX.xf files. Tomograms that were used for STA are marked in tomo_list_1degree.txt. Coordinates to raw ribosome particle picks by template matching are provided in TM_1degree_threshold5.star and TM_1degree_threshold6.star. Ribosome particle locations after M refinment are provided in M_1degree_particles.star.

Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 2-degree tilt increment /data/Data_2degree tilt series MRC MRC 119 1 SIGNED 16 BIT INTEGER 4096 1.971 4096 1.971

Tilt-series were aimed to collect 61 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided. Tilt images that were discarded during processing are specified in "tilt_clean_2deg.txt". Alignment results from IMOD are provided as TS_XXXX.xf files. Tomograms that were used for STA are marked in tomo_list_2degree.txt. Coordinates to raw ribosome particle picks by template matching are provided in TM_2degree_threshold5.star and TM_2degree_threshold6.star. Ribosome particle locations after M refinment are provided in M_2degree_particles.star.

Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 3-degree tilt increment /data/Data_3degree tilt series MRC MRC 120 1 SIGNED 16 BIT INTEGER 4096 1.971 4096 1.971

Tilt-series were aimed to collect 41 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided. Tilt images that were discarded during processing are specified in "tilt_clean_3deg.txt". Alignment results from IMOD are provided as TS_XXXX.xf files. Tomograms that were used for STA are marked in tomo_list_3degree.txt. Coordinates to raw ribosome particle picks by template matching are provided in TM_3degree_threshold5.star and TM_3degree_threshold5.5.star. Ribosome particle locations after M refinment are provided in M_3degree_particles.star.

Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 5-degree tilt increment /data/Data_5degree tilt series MRC MRC 122 1 SIGNED 16 BIT INTEGER 4096 1.971 4096 1.971

Tilt-series were aimed to collect 25 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided. Tilt images that were discarded during processing are specified in "tilt_clean_5deg.txt". Alignment results from IMOD are provided as TS_XXXX.xf files. Tomograms that were used for STA are marked in tomo_list_5degree.txt. Coordinates to raw ribosome particle picks by template matching are provided in TM_5degree_threshold5.star. Ribosome particle locations after M refinment are provided in M_5degree_particles.star.

Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 10-degree tilt increment /data/Data_10degree tilt series MRC MRC 89 1 SIGNED 16 BIT INTEGER 4096 1.971 4096 1.971

Tilt-series were aimed to collect 13 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided. Tilt images that were discarded during processing are specified in "tilt_clean_10deg.txt". Alignment results from IMOD are provided as TS_XXXX.xf files. Tomograms that were used for STA are marked in tomo_list_10degree.txt. Coordinates to raw ribosome particle picks by template matching are provided in TM_10degree_threshold3.75.star. Ribosome particle locations after M refinment are provided in M_10degree_particles.star.