REL
2026-03-18
2026-04-08
2026-04-08
Cryo-EM micrographs of phosphorylated AP2 bound to the endocytic regulator NECAP
0000-0003-1136-6000
Richard
Wayne
Baker
University of North Carolina at Chapel Hill
3049E Genetic Medicine Bldg
Chapel Hill
NC
United States
27599
0000-0003-1136-6000
Gunther
Hollopeter
Cornell University
Ithaca
NY
United States
14853
Partlow EA
Baker RW
Leschziner AE
Hollopeter G
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM127548
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM127548-01A1
United States
Damon Runyon Cancer Research Foundation
DRG-#2285-17/
United States
National Institutes of Health (NIH)
S10 OD018516
United States
2.6
10.6019/EMPIAR-13389
EMDB
molecule
EMD-20215
EMD-20220
Partlow EA
Baker RW
Leschziner AE
Hollopeter G
A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex
eLife
Elife
8
2019
English
10.7554/elife.50003
31464684
Raw, unaligned movies of phosphorylated AP2 in three distinct states.
Phosphorylated AP2
Phosphorylated AP2 bound to NECAP
Phosphorylated AP2 bound to NECAP and DNA
Raw, unaligned cryo-EM micrographs of phosphorylated AP-2
/data/pAP2
micrographs - multiframe
TIFF
TIFF
542
60
UNSIGNED BYTE
7676
0.58
7420
0.58
Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2). Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in super-resolution mode.
200 keV Talos Arctica cryoTEM
Images were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode
exposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2
Cs - 2.7 mm
Ac - 0.1
Raw, unaligned cryo-EM micrographs of phosphorylated AP-2 bound to NECAP
/data/pAP2+NECAP
micrographs - multiframe
TIFF
TIFF
2860
60
UNSIGNED BYTE
7676
0.58
7420
0.58
Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2) bound to the endocytic regulator NECAP. Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in super-resolution mode.
200 keV Talos Arctica cryoTEM
Images were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode
exposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2
Cs - 2.7 mm
Ac - 0.1
Raw, unaligned cryo-EM micrographs of phosphorylated AP-2 bound to NECAP in the presence of DNA
/data/pAP2+NECAP+DNA
micrographs - multiframe
TIFF
TIFF
2314
60
UNSIGNED BYTE
3838
1.16
3710
1.16
Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2) bound to the endocytic regulator NECAP in the presence of DNA. Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in counting mode.
200 keV Talos Arctica cryoTEM
Images were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode
exposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2
Cs - 2.7 mm
Ac - 0.1