REL 2025-09-12 2026-03-16 2026-03-16 0000-0001-5582-2851 Mariusz Czarnocki-Cieciura International Institute of Molecular and Cell Biology in Warsaw 4 Ks. Trojdena Street Warsaw Poland 02-106 0000-0001-8632-0977 Marcin Nowotny International Institute of Molecular and Cell Biology in Warsaw 4 Ks. Trojdena Street Warsaw Poland 02-109 Chamera S Zajko W Czarnocki-Cieciura M Jaciuk M Koziej Ł Nowak J Wycisk K Sroka M Chramiec-Głąbik A Śmietański M Gołębiowski F Warmiński M Jemielity J Glatt S Nowotny M Polish National Science Centre 2019/33/B/NZ1/02839 Poland Other government 1/SOL/2021/2 Poland European Research Council (ERC) 101001394 Poland 3.9 10.6019/EMPIAR-13357 EMDB molecule EMD-50108 Chamera S Zajko W Czarnocki-Cieciura M Jaciuk M Koziej Ł Nowak J Wycisk K Sroka M Chramiec-Głąbik A Śmietański M Gołębiowski F Warmiński M Jemielity J Glatt S Nowotny M The Journal of biological chemistry J Biol Chem 5 301 2025 English 10.1016/j.jbc.2025.108506 40220997 Unaligned multi-frame movies of Danio rerio tRNA ligase complex (full-length version) /data/Movies micrographs - multiframe TIFF TIFF 10703 40 UNSIGNED BYTE 5760 0.86 4092 0.86

Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 39.41 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm.

data/Movies/*.tiff data/particles/EMD-50108_particlestack.star data/particles Aligned particles of Danio rerio tRNA ligase complex (full-length version) /data/particles picked particles - single frame - processed MRCS MRCS 35251 1 32 BIT FLOAT 320 0.86 320 0.86

Aligned particles with corresponding Relion-3 STAR file. Micrographs were initially binned 2x during Motion correction.

data/Movies/*.tiff data/particles/EMD-50108_particlestack.star data/particles