REL 2025-11-06 2026-04-09 2026-04-09 0009-0001-4737-542X Madeleine Camille Duquette UC San Diego 9500 Gilman Drive La Jolla CA United States 92093 0000-0003-4677-9809 Elizabeth Villa Department of Molecular Biology, University of California San Diego, La Jolla, CA, USA 9500 Gilman Drive La Jolla CA United States 92093 Duquette MC National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) U54 AI170856 United States 963.0 10.6019/EMPIAR-13331 EMDB virus EMD-75641 Shehata M Casalino L Duquette M Chen S Flaherty A Villa E Amaro RE bioRxiv United States 2025 English 10.1101/2025.03.26.645577

The micrographs were collected on a Titan Krios G4 TEM (Thermo Fisher) operating at 300 Kv with a Falcon 4i direct electron detector (Thermo Fisher) at a nominal magnification of 81,000x (pixel size 1.561 Å, counting mode). A Selectris X energy filter with a 10 eV-wide slit was used throughout data collection. PaceTOMO was used to collect tilt series. 224 EER frames were collected per tilt series at a dose rate of 4.4 e-/Å2/s. The raw EER frames are provided, as well as the mdocs.

Raw cryoET data from collection on 7/18/2025 /data/Dataset1_frames micrographs - multiframe EER EER 4012 224 UNSIGNED 16 BIT INTEGER 4096 1.561 4096 1.561

There are 224 EER frames per image, and each tilt series is comprised of up to 37 tilts. Some of them have fewer tilts because the stage has a maximum tilt angle and some of the targets had a larger estimated pre-tilt while setting up paceTOMO. Associated metadata from SerialEM is in the mdocs folder.

Raw cryoET data from collection on 7/19/2025 /data/Dataset2_frames micrographs - multiframe EER EER 5884 224 UNSIGNED 16 BIT INTEGER 4096 1.561 4096 1.561

There are 224 EER frames per image, and each tilt series is comprised of up to 37 tilts. Some of them have fewer tilts because the stage has a maximum tilt angle and some of the targets had a larger estimated pre-tilt while setting up paceTOMO. Associated metadata from SerialEM is in the mdocs folder.