Cryo-electron tomography of the actin cortex of NIH 3T3 fibroblasts upon regulation of membrane-to-cortex attachment

REL 2025-12-08 2026-05-13 2026-05-13 Cryo-electron tomography of the actin cortex of NIH 3T3 fibroblasts upon regulation of membrane-to-cortex attachment 0000-0002-2842-0360 Samuel François Gerard EMBL Heidelberg Germany 69117 0000-0001-6968-041X Julia Mahamid EMBL Heidelberg Germany 69117 0000-0001-6864-8901 Alba Diz-Munoz EMBL Heidelberg Germany 69117 Gérard SF Cheng DCW Toro-Nahuelpan M Mahamid J Diz-Munoz A European Research Council (ERC) 101124221 Germany German Research Foundation (DFG) DI 2205/3-1 Germany Human Frontier Science Program (HFSP) RGY0073/2018 Germany EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND 847532 Germany EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND 664726 Germany European Molecular Biology Organization (EMBO) ALTF-2024 Germany Boehringer Ingelheim Fonds (BIF) - Germany 804.3 10.6019/EMPIAR-13326 EMDB cell EMD-56367 Strauss L Lembo S Gérard SF Siggel M Cheng DCW Bergert M Foster SK Vermeil J Toro-Nahuelpan M Fischer L Yu Q Sitarska E Chan CJ Kosinski J Piel M Du Roure O Heuvingh J Mahamid J Diz-Munoz A The membrane-to-cortex distance gates mDia1 activity to control cortical mechanics 10.1038/s41467-026-72845-3 Raw unaligned multi-frame micrographs for each tilt in 17 tilt-series in iMC control condition /data/iMC_control/frames micrographs - multiframe TIFF TIFF 1012 10 UNSIGNED 16 BIT INTEGER variable 3.37 variable 3.37

Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks with a serialEM plugin or in Warp (.mrc or .st files, respectively) located in the processing folders. Naming for tomograms of iMC control cells: TS_01_iMC_control, TS_02_iMC_control, TS_03_iMC_control, TS_04_iMC_control, TS_05_iMC_control, TS_06_iMC_control, TS_07_iMC_control, TS_08_iMC_control, TS_15_iMC_control, TS_16_iMC_control, TS_17_iMC_control, TS_18_iMC_control, TS_19_iMC_control, TS_20_iMC_control, TS_21_iMC_control, TS_23_iMC_control, TS_24_iMC_control. Metadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.

Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in iMC control conditions /data/iMC_control/tomograms reconstructed volumes MRC MRC 17 1 32 BIT FLOAT variable 13.48 variable 13.48

Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.mrc or .st files located in processing folders, .tif files in frames folders). Metadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders. Naming for tomograms of iMC control cells: TS_01_iMC_control, TS_02_iMC_control, TS_03_iMC_control, TS_04_iMC_control, TS_05_iMC_control, TS_06_iMC_control, TS_07_iMC_control, TS_08_iMC_control, TS_15_iMC_control, TS_16_iMC_control, TS_17_iMC_control, TS_18_iMC_control, TS_19_iMC_control, TS_20_iMC_control, TS_21_iMC_control, TS_23_iMC_control, TS_24_iMC_control. For each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.

Raw unaligned multi-frame micrographs for each tilt in 9 tilt-series in iMC induced condition /data/iMC_induced/frames micrographs - multiframe TIFF TIFF 524 10 UNSIGNED 16 BIT INTEGER variable 3.37 variable 3.37

Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders. Naming for tomograms of iMC induced cells: TS_09_iMC_induced, TS_10_iMC_induced, TS_11_iMC_induced, TS_12_iMC_induced, TS_13_iMC_induced, TS_14_iMC_induced, TS_25_iMC_induced, TS_26_iMC_induced, TS_28_iMC_induced. Metadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.

Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in iMC induced conditions /data/iMC_induced/tomograms reconstructed volumes MRC MRC 9 1 32 BIT FLOAT variable 13.48 variable 13.48

Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders). Metadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders. Naming for tomograms of iMC induced cells: TS_09_iMC_induced, TS_10_iMC_induced, TS_11_iMC_induced, TS_12_iMC_induced, TS_13_iMC_induced, TS_14_iMC_induced, TS_25_iMC_induced, TS_26_iMC_induced, TS_28_iMC_induced. For each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.

Raw unaligned multi-frame micrographs for each tilt in 6 tilt-series in CAEzrin control condition /data/CAEzrin_control/frames micrographs - multiframe TIFF TIFF 349 10 UNSIGNED 16 BIT INTEGER variable 3.37 variable 3.37

Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders. Naming for tomograms of CAEzrin control cells: TS_43_CAEz_control, TS_45_CAEz_control, TS_47_CAEz_control, TS_48_CAEz_control, TS_49_CAEz_control, TS_50_CAEz_control. Metadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.

Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in CAEzrin control conditions /data/CAEzrin_control/tomograms reconstructed volumes MRC MRC 6 1 32 BIT FLOAT variable 13.48 variable 13.48

Raw unaligned multi-frame micrographs for each tilt in 20 tilt-series in CAEzrin induced condition /data/CAEzrin_control/frames micrographs - multiframe TIFF TIFF 1041 10 UNSIGNED 16 BIT INTEGER variable 3.37 variable 3.37

Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks with a serialEM plugin or in Warp (.mrc or .st files, respectively) located in the processing folders. Naming for tomograms of CAEzrin induced cells: TS_35_CAEz_induced, TS_36_CAEz_induced, TS_37_CAEz_induced, TS_38_CAEz_induced, TS_39_CAEz_induced, TS_40_CAEz_induced, TS_41_CAEz_induced, TS_42_CAEz_induced, TS_51_CAEz_induced, TS_52_CAEz_induced, TS_53_CAEz_induced, TS_54_CAEz_induced, TS_55_CAEz_induced, TS_56_CAEz_induced, TS_57_CAEz_induced, TS_58_CAEz_induced, TS_59_CAEz_induced, TS_60_CAEz_induced, TS_61_CAEz_induced, TS_62_CAEz_induced. Metadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.

Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in CAEzrin induced conditions /data/CAEzrin_induced/tomograms reconstructed volumes MRC MRC 20 1 32 BIT FLOAT variable 13.48 variable 13.48

Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders). Metadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders. Naming for tomograms of CAEzrin induced cells: TS_35_CAEz_induced, TS_36_CAEz_induced, TS_37_CAEz_induced, TS_38_CAEz_induced, TS_39_CAEz_induced, TS_40_CAEz_induced, TS_41_CAEz_induced, TS_42_CAEz_induced, TS_51_CAEz_induced, TS_52_CAEz_induced, TS_53_CAEz_induced, TS_54_CAEz_induced, TS_55_CAEz_induced, TS_56_CAEz_induced, TS_57_CAEz_induced, TS_58_CAEz_induced, TS_59_CAEz_induced, TS_60_CAEz_induced, TS_61_CAEz_induced, TS_62_CAEz_induced. For each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.

Raw unaligned multi-frame micrographs for each tilt in 8 tilt-series in 6FP-iMC control condition /data/6FP_control/frames micrographs - multiframe TIFF TIFF 406 10 UNSIGNED 16 BIT INTEGER variable 3.37 variable 3.37

Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders. Naming for tomograms of 6FP-iMC control cells: TS_90_6FP_control, TS_91_6FP_control, TS_94_6FP_control, TS_95_6FP_control, TS_96_6FP_control, TS_103_6FP_control, TS_104_6FP_control, TS_105_6FP_control. Metadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.

Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in 6FP-iMC control conditions /data/6FP_control/tomograms reconstructed volumes MRC MRC 8 1 32 BIT FLOAT variable 13.48 variable 13.48

Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders). Metadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders. Naming for tomograms of 6FP-iMC control cells: TS_90_6FP_control, TS_91_6FP_control, TS_94_6FP_control, TS_95_6FP_control, TS_96_6FP_control, TS_103_6FP_control, TS_104_6FP_control, TS_105_6FP_control. For each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.

Raw unaligned multi-frame micrographs for each tilt in 10 tilt-series in 6FP-iMC induced condition /data/6FP_induced/frames micrographs - multiframe TIFF TIFF 539 10 UNSIGNED 16 BIT INTEGER variable 3.37 variable 3.37

Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders. Naming for tomograms of 6FP-iMC induced cells: TS_106_6FP_induced, TS_107_6FP_induced, TS_109_6FP_induced, TS_110_6FP_induced, TS_111_6FP_induced, TS_113_6FP_induced, TS_114_6FP_induced, TS_116_6FP_induced, TS_117_6FP_induced, TS_118_6FP_induced. Metadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.

Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in 6FP-iMC induced conditions /data/6FP_induced/tomograms reconstructed volumes MRC MRC 10 1 32 BIT FLOAT variable 13.48 variable 13.48

Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders). Metadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders. Naming for tomograms of 6FP-iMC induced cells: TS_106_6FP_induced, TS_107_6FP_induced, TS_109_6FP_induced, TS_110_6FP_induced, TS_111_6FP_induced, TS_113_6FP_induced, TS_114_6FP_induced, TS_116_6FP_induced, TS_117_6FP_induced, TS_118_6FP_induced. For each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.