REL
2026-02-14
2026-03-12
2026-03-12
cryo electron tomography of mouse hippocampal CA1-py dataset
0000-0002-2197-2357
Calina
Glynn
Rosalind Franklin Institute
Building R113, Rutherford Appleton Laboratory, Harwell Campus
Didcot
Oxfordshire
United Kingdom
ox11 0qx
0000-0002-2197-2357
Calina
Glynn
Rosalind Franklin Institute
Building R113, Rutherford Appleton Laboratory, Harwell Campus
Didcot
oxfordshire
United Kingdom
OX11 0QX
Glynn CG
Smith JLRS
Wellcome Trust
225902/Z/22/Z
United Kingdom
Wellcome Trust
220526/Z/20/Z
United Kingdom
Wellcome Trust
226810/Z/22/Z
United Kingdom
175.8
10.6019/EMPIAR-13303
EMDB
tissue
EMD-55442
Glynn C
Smith JLR
Case M
Csöndör R
Katsini A
Sanita ME
Glen TS
Pennington A
Grange M
A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue
Cell reports methods
Cell Rep Methods
United States
7
5
2025
English
10.1016/j.crmeth.2025.101080
40527314
Raw eer, mrc, and mdocs and reconstructions of 35 tilt series with 41 total tilts and 130 e-/A2 per position. Hippocampal CA1-sp dataset. Right hemisphere of a 172 day old female mouse. 100 micron thick slice incubation for 20 minutes incubation with cryo protectant 10% dextran 10% sucrose dissolved in NMDG buffer pH 7.4. Tilt series collected at 64000x. Thick lamella, low quality tilt series.
EMPIAR-13281
EMPIAR-13287
EMPIAR-13288
EMPIAR-13289
EMPIAR-13301
EMPIAR-13302
EMPIAR-13304
EMPIAR-13314
raw eer of tilt series
/data/eer
tilt series
EER
EER
35
1
UNSIGNED BYTE
4096
1.98
4096
1.98
35 tilt series comprised of 41 tilts with 3 degree spacing between tilts in eer format. Total dose 130e-/A^2 / tilt series for 3.17e-/A^2 / tilt. Data acquired at 64000x with 1.98A / pixel. Thick lamella.
raw .mrc of tilt series
/data/tilt_mrc
tilt series
MRC
MRC
35
1
32 BIT FLOAT
4096
1.98
4096
1.98
35 tilt series comprised of 41 tilts with 3 degree spacing between tilts in mrc format. Total dose 130e-/A^2 / tilt series for 3.17e-/A^2 / tilt. Data acquired at 64000x with 1.98A / pixel. Thick lamella.
unfiltered bin 8 reconstructions
/data/tomos_unfiltered_bin8
reconstructed volumes
MRC
MRC
35
1
32 BIT FLOAT
512
1.98
512
1.98
Unfiltered nondenoised reconstructed tomograms from 35 tilt series carried out in aretomo2. Thick lamella.
isonet deconvolved bin8 tomograms
/data/tomos_isonetdeconv_bin8
reconstructed volumes
MRC
MRC
35
1
32 BIT FLOAT
512
15.84
512
15.84
unfiltered reconstructed tomograms deconvolved (but not missing wedge corrected) in isonet, bin8. --snrfalloff 0.7 --deconvstrength 1.1. Thick lamella.