REL 2026-01-28 2026-07-12 2026-07-12 0000-0003-1981-1244 Marcell Zimanyi Department of Biochemistry and Biophysics, University of California San Francisco San Francisco CA United States 94158 0000-0001-7704-9185 Charles S Craik Department of Pharmaceutical Chemistry, University of California San Francisco San Francisco CA United States 94158 0000-0001-9535-0369 Yifan Cheng Department of Biochemistry and Biophysics, University of California San Francisco San Francisco CA United States 94158 Zimanyi M Hulce KR Bohn M Norman J Rohweder PJ Cheng Y Craik CS Detomasi TC National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) U54AI170792 United States 7.4 10.6019/EMPIAR-13274 EMDB molecule EMD-72658 EMD-72659 EMD-72660 Zimanyi M Hulce KR Bohn M Norman J Rohweder PJ Cheng Y Craik CS Detomasi TC bioRxiv 10.1101/2025.10.17.683123 Unaligned multi-frame micrographs of human cytomegalovirus protease in complex with an inhibitory antibody fragment /data/movies micrographs - multiframe EER EER 16378 1096 32 BIT FLOAT variable 0.743 variable 0.743

There are movies from 2 different grids in this dataset that were collected in a single krios session. In MZJ_1, the grids are Quantifoil 1.2/1.3 400 mesh copper foil grids, and MZJ_3 are UltrAUfoil 1.2/1.3 300 mesh grids. The MZJ_3 dataset tends to yield better particles, and our final reconstructions were comprised of about 80 percent particles from MZJ_3.

Unclassified particles of human cytomegalovirus protease bound to an inhibitory antibody fragment /data/All_particles_unsorted picked particles - single frame - processed MRC MRC 1455383 1 32 BIT FLOAT variable variable variable variable

All particles used in final reconstructions. Upon classification, at least 3 poses of the protease relative to the Fab can be resolved.