Cryo-electron tomography of microtubules in axons from human cortical neurons derived from induced pluripotent stem cells (iPSCs).

REL 2025-12-08 2026-03-13 2026-04-15 Cryo-electron tomography of microtubules in axons from human cortical neurons derived from induced pluripotent stem cells (iPSCs). 0000-0003-2621-7307 Antonina Roll-Mecak National Institutes of Health Cell Biology and Biophysics Unit, NINDS Porter Neuroscience Research Center Building 35, Room 3B-205 35 Convent Drive Bethesda Maryland United States 20814 0000-0003-2621-7307 Antonina Roll-Mecak National Institutes of Health Cell Biology and Biophysics Unit, NINDS Porter Neuroscience Research Center Building 35, Room 3B-205 35 Convent Drive Bethesda Maryland United States 20814 Zehr EA Roll-Mecak A National Institutes of Health (NIH) 1ZIANS003164 United States 544.4 10.6019/EMPIAR-13239 EMDB cell EMD-70956 Zehr EA Sun S Sarbanes SL Roll-Mecak A Microtubules in the axon are GDP-bound but adopt a stable GTP-like expanded state. Nature Structural & Molecular Biology NSMB United States 2026 English 10.1038/s41594-026-01787-7 N/A Reconstructed tomograms of axons of human cortical neurons derived from iPSCs /data reconstructed volumes MRC MRC 5 1200 1 1200 32 BIT FLOAT 4092 2.656 5760 2.656

Reconstructed tomograms containing microtubules in axons of human cortical neurons derived from iPSCs. The tomograms were reconstructed using AreTomo2 and binned 2 times. Microtubule lattice spacing analysis was subsequently performed.