REL
2026-01-01
2026-02-11
2026-02-11
Unaligned Movies of E. coli grown at 25°C clarified cell lysate
0000-0002-4545-4001
Mira
May
Department of Biology, MIT
31 Ames St
Cambridge
MA
United States
02139
0000-0002-8858-8907
Joseph
Davis
Department of Biology, MIT
31 Ames St
Cambridge
MA
United States
02139
May MB
Lopez Perez GS
Davis JH
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01GM144542
United States
National Science Foundation (NSF, United States)
CAREER-2046778
United States
2.0
10.6019/EMPIAR-13235
EMDB
cell
EMD-72030
May MB
Lopez Perez GS
Davis JH
Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states
Proceedings of the National Academy of Sciences (PNAS)
10.1101/2025.08.21.669550
40894545
Name: Unaligned Movies of E. coli grown at 25°C clarified cell lysate
/data
micrographs - multiframe
TIFF
TIFF
7908
45
UNSIGNED BYTE
5760
1.06
4092
1.06
Nominal Defocus range (min, max, step): -0.03,-0.2 to -2 in steps of 0.2
Total exposure on specimen: 45.1838 el/A2
Exposure time: 3.15 s
Nominal pixel size: 1.06 A
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3900 V
Microscope / Detector: Krios G3i / K3
data/*_fractions.tiff