<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13231" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-01-20</depositionDate>
            <releaseDate>2026-02-03</releaseDate>
            <updateDate>2026-02-03</updateDate>
        </keyDates>
        <title>Single Particle Raw Images for nicotinic receptor alpha 7 in complex with epibatidine and PNU</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-0055-1019</authorORCID>
            <firstName>Colleen</firstName>
            <middleName>Marcy</middleName>
            <lastName>Noviello</lastName>
            <organization type="academic">University of California, San Diego</organization>
            <street>Eucalyptus Lane</street>
            <townOrCity>La Jolla</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>92093</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-4283-1147</authorORCID>
            <firstName>Ryan</firstName>
            <lastName>Hibbs</lastName>
            <organization type="academic">University of California, San Diego</organization>
            <street>Eucalyptus Lane</street>
            <townOrCity>La Jolla</townOrCity>
            <country>United States</country>
            <postOrZipCode>92093</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-0055-1019">Noviello CM</author>
            <author authorORCID="0000-0003-4283-1147">Hibbs RE</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)</fundingBody>
                <code>NS095899</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">7.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-13231</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-22983</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-0055-1019" order="1">Noviello CM</author>
                    <author authorORCID="0000-0003-4283-1147" order="2">Hibbs RE</author>
                    <title>Structure and gating mechanism of the α7 nicotinic acetylcholine receptor</title>
                    <journal>Cell</journal>
                    <journalAbbreviation>Cell</journalAbbreviation>
                    <country></country>
                    <issue>8</issue>
                    <volume>184</volume>
                    <firstPage>2121</firstPage>
                    <lastPage>2.134e+16</lastPage>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.cell.2021.02.049</externalReferences>
                    <externalReferences type="pubmed">33735609</externalReferences>
                    <details>Raw micrograph stacks</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>unaligned raw tiffs of epibatidine + PNU nicotinic receptor alpha 7 complex in nanodisc</name>
        <directory>/data/CMN_PNU/July_2019</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2809</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>0.83 is Angstrom/pixel. Note there are two datasets, one from July 2019 and one from June 2020. Both were collected on the same setup and can be merged.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>unaligned raw tiffs of epibatidine + PNU nicotinic receptor alpha 7 complex in nanodisc</name>
        <directory>/data/CMN_PNU/June_2020/CN_PNU2/movies2020June17</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>13940</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>same as above</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
