<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13180" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-12-19</depositionDate>
            <releaseDate>2026-04-16</releaseDate>
            <updateDate>2026-04-16</updateDate>
        </keyDates>
        <title>Dimer of PglZ in complex with BrxB-BrxC fusion from the Acinetobacter BREX system</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-0008-473X</authorORCID>
            <firstName>Lindsey</firstName>
            <lastName>Doyle</lastName>
            <organization type="academic">Fred Hutch Cancer Center</organization>
            <street>1100 Fairview Ave N</street>
            <townOrCity>Seattle</townOrCity>
            <stateOrProvince>Washington</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>98109</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-0339-0035</authorORCID>
            <firstName>Brett</firstName>
            <middleName>K</middleName>
            <lastName>Kaiser</lastName>
            <organization type="academic">Department of Biology, Seattle University</organization>
            <street>901 12th Ave</street>
            <townOrCity>Seattle</townOrCity>
            <stateOrProvince>WA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>98122</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-5174-7858</authorORCID>
            <firstName>Barry</firstName>
            <middleName>L</middleName>
            <lastName>Stoddard</lastName>
            <organization type="academic">Division of Basic Sciences, Fred Hutchinson Cancer Center</organization>
            <street>1100 Fairview Ave N</street>
            <townOrCity>Seattle</townOrCity>
            <stateOrProvince>WA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>98109</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-0008-473X">Doyle LA</author>
            <author authorORCID="0000-0001-5174-7858">Stoddard BL</author>
            <author authorORCID="0000-0003-0339-0035">Kaiser BK</author>
            <author authorORCID="0009-0001-3643-1721">Kaiser AJ</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R35 GM148166</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R15 GM140375</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Other private</fundingBody>
                <code>N/A</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">3.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-13180</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-74435</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0002-0008-473X" order="1">Doyle LD</author>
                    <title>Competing forms of protein-protein association and DNA binding exhibited by BrxC from the BREX phage restriction system</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of BrxB-BrxC fusion in complex with PglZ</name>
        <directory>/data/RawData</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>4008</numImagesOrTiltSeries>
        <framesPerImage>100</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>100</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.561</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.561</pixelHeight>
        </dimensions>
        <details>The data is raw, multi-frame movies without any correction applied. Collected with SerialEM in LZW compressed TIFFs. The gain reference in MRC format is included in the data directory and should be flipped along the Y axis. 200 kV, Cs 2.7, total exposure dose 50 e/A^2.</details>
        <segmentationList/>
        <micrographsFilePattern>data/RawData/20241003_LD10-2_*.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
