FIB-SEM imaging of control HeLa cells, cells expressing WT STARD3, and cells expressing the STARD3 FA/YA mutant

REL 2025-12-04 2026-02-26 2026-03-02 FIB-SEM imaging of control HeLa cells, cells expressing WT STARD3, and cells expressing the STARD3 FA/YA mutant 0000-0002-0526-0720 Fabien ALPY IGBMC 1 rue Laurent Fries ILLKIRCH France 67400 0000-0002-0526-0720 Fabien Alpy IGBMC 1 rue Laurent Fries ILLKIRCH France 67400 Eichler J Hanss V Alpy F Agence Nationale de la Recherche (ANR) ANR-25-CE13-4997 France Agence Nationale de la Recherche (ANR) ANR-22-CE44-0019 France 10.3 10.6019/EMPIAR-13156 FIB-SEM cell Eichler J Wendling C Huver S Zouiouich M Hanss V Cardinal A Fimbel V Birck C McEwen AG Knorr C Fromental-Ramain C Boutry M Chenard MP Drin G Tomasetto C Alpy F STARD3 regulates lysosome positioning and contacts via a GSK3-controlled phosphorylation switch The EMBO journal EMBO J 2026 English 10.1038/s44318-026-00705-3 41741634 HeLa/Ctrl /data/HeLaWT_Raw reconstructed volumes TIFF TIFF 994 1 UNSIGNED BYTE 2768 80.0 1188 80.0

data/HeLaWT_Raw/ER.tif Whole-image Endoplasmic Reticulum segmentation (excluding the ROI) TIFF data/HeLaWT_Raw/Endosomes.tif Whole-image Endosome/Lysosome segmentation (excluding the ROI) data/HeLaWT_Raw/Mitochondria.tif Whole-image Mitochondria segmentation (excluding the ROI) data/HeLaWT_Raw/ER_ROI.tif Endoplasmic Reticulum segmentation within the ROI ROI starting position (1536, 64, 5), ROI size 1200 × 900 × 444 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. data/HeLaWT_Raw/Endosomes_ROI.tif Endosome/Lysosome segmentation within the ROI ROI starting position (1536, 64, 5), ROI size 1200 × 900 × 444 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. data/HeLaWT_Raw/Mitochondria_ROI.tif Mitochondria segmentation within the ROI ROI starting position (1536, 64, 5), ROI size 1200 × 900 × 444 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. HeLa/STARD3 /data/HeLaSTARD3_WT_Raw reconstructed volumes TIFF TIFF 810 1 UNSIGNED BYTE 2418 50.0 2748 50.0

data/HeLaSTARD3_WT_Raw/ER.tif Whole-image Endoplasmic Reticulum segmentation (excluding the ROI) data/HeLaSTARD3_WT_Raw/Endosomes.tif Whole-image Endosome/Lysosome segmentation (excluding the ROI) data/HeLaSTARD3_WT_Raw/Mitochondria.tif Whole-image Mitochondria segmentation (excluding the ROI) data/HeLaSTARD3_WT_Raw/ER_ROI.tif Endoplasmic Reticulum segmentation within the ROI ROI starting position (16, 151, 428), ROI size 1400 × 1100 × 382 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. data/HeLaSTARD3_WT_Raw/Endosomes_ROI.tif Endosome/Lysosome segmentation within the ROI ROI starting position (16, 151, 428), ROI size 1400 × 1100 × 382 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. data/HeLaSTARD3_WT_Raw/Mitochondria_ROI.tif Mitochondria segmentation within the ROI ROI starting position (16, 151, 428), ROI size 1400 × 1100 × 382 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. HeLa/STARD3 FA/YA /data/HeLaSTARD3_FA_YA_Raw reconstructed volumes TIFF TIFF 600 1 UNSIGNED BYTE 2516 80.0 1092 80.0

data/HeLaSTARD3_FA_YA_Raw/ER.tif Whole-image Endoplasmic Reticulum segmentation (excluding the ROI) data/HeLaSTARD3_FA_YA_Raw/Endosomes.tif Whole-image Endosome/Lysosome segmentation (excluding the ROI) data/HeLaSTARD3_FA_YA_Raw/Mitochondria.tif Whole-image Mitochondria segmentation (excluding the ROI) data/HeLaSTARD3_FA_YA_Raw/ER_ROI.tif Endoplasmic Reticulum segmentation within the ROI ROI starting position (386, 50, 120), ROI size 700 × 700 × 449 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. data/HeLaSTARD3_FA_YA_Raw/Endosomes_ROI.tif Endosome/Lysosome segmentation within the ROI ROI starting position (386, 50, 120), ROI size 700 × 700 × 449 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels. data/HeLaSTARD3_FA_YA_Raw/Mitochondria_ROI.tif Mitochondria segmentation within the ROI ROI starting position (386, 50, 120), ROI size 700 × 700 × 449 px (X × Y × Z). ROI coordinates are in the ImageJ system (X, Y, Z), with (0,0,0) at the top-left pixel of the first slice. Each ROI is defined by its starting position (top-left pixel of the first slice in the ROI) and size (X × Y × Z) in pixels.