<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13136" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-09-04</depositionDate>
            <releaseDate>2026-01-11</releaseDate>
            <updateDate>2026-01-11</updateDate>
        </keyDates>
        <title>Electron cryomicroscopy of NSF, the binary SNARE complex of SNAP-25 and syntaxin-1a, and alpha-SNAP under non-hydrolyzing conditions</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-8182-3655</authorORCID>
            <firstName>Kristopher</firstName>
            <middleName>Ian</middleName>
            <lastName>White</lastName>
            <organization type="academic">Department of Molecular and Cellular Physiology, Stanford University, and the Howard Hughes Medical Institute</organization>
            <street>318 Campus Drive</street>
            <townOrCity>Stanford</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94305</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-5121-2036</authorORCID>
            <firstName>Axel</firstName>
            <lastName>Brunger</lastName>
            <organization type="academic">Department of Molecular and Cellular Physiology, Stanford University, and the Howard Hughes Medical Institute</organization>
            <street>318 Campus Drive</street>
            <townOrCity>Stanford</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94305</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-8182-3655">White KI</author>
            <author authorORCID="0000-0001-5121-2036">Brunger AT</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Howard Hughes Medical Institute (HHMI)</fundingBody>
                <code>None</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>RO1MH63105</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">14.6</datasetSize>
        <entryDOI>10.6019/EMPIAR-13136</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-70547</emdbEntry>
            <emdbEntry>EMD-70548</emdbEntry>
            <emdbEntry>EMD-70550</emdbEntry>
            <emdbEntry>EMD-71437</emdbEntry>
            <emdbEntry>EMD-71438</emdbEntry>
            <emdbEntry>EMD-71458</emdbEntry>
            <emdbEntry>EMD-71478</emdbEntry>
            <emdbEntry>EMD-71498</emdbEntry>
            <emdbEntry>EMD-71496</emdbEntry>
            <emdbEntry>EMD-70553</emdbEntry>
            <emdbEntry>EMD-71475</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-8182-3655" order="1">White KI</author>
                    <author authorORCID="0000-0003-0201-2796" order="2">Khan YA</author>
                    <author order="3">Qiu K</author>
                    <author authorORCID="0000-0002-0075-4203" order="4">Balaji A</author>
                    <author authorORCID="0000-0002-5186-4648" order="5">Couoh-Cardel S</author>
                    <author order="6">Esquivies L</author>
                    <author order="7">Pfuetzner RA</author>
                    <author authorORCID="0000-0003-4288-3203" order="8">Diao J</author>
                    <author authorORCID="0000-0001-5121-2036" order="9">Brunger AT</author>
                    <title>Structural remodeling of target-SNARE protein complexes by NSF enables synaptic transmission</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>16</volume>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-025-62764-0</externalReferences>
                    <externalReferences type="pubmed">40993127</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of NSF, the binary SNARE complex of SNAP-25 and syntaxin-1a, and alpha-SNAP under non-hydrolyzing conditions</name>
        <directory>/data/20190624_Micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>12483</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details>0.789 e/A2</details>
        <segmentationList/>
        <micrographsFilePattern>data/20190624_Micrographs/20190624_20s-ss17_129-1_00001_Jun24_12.29.31.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Gain reference, 20190624</name>
        <directory>/data</directory>
        <category>micrographs - single frame</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/20190624_gain-ref.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame micrographs of NSF, the binary SNARE complex of SNAP-25 and syntaxin-1a, and alpha-SNAP under non-hydrolyzing conditions</name>
        <directory>/data/20190919_Micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>13570</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details>0.919 e/A2</details>
        <segmentationList/>
        <micrographsFilePattern>data/20190919_Micrographs/20190920_20s-ss17-m18p335a_318-1_00001_Sep20_14.12.27.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Gain Reference, 20190919</name>
        <directory>/data</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/20190919_gain-ref.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame micrographs of NSF, the binary SNARE complex of SNAP-25 and syntaxin-1a, and alpha-SNAP under non-hydrolyzing conditions</name>
        <directory>/data/20191216_Micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7818</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/20191216_Micrographs/20191216_20s-binary-atpedta-hiaSNAP_147-1_00001_Dec16_12.48.02.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Gain reference, 20191216</name>
        <directory>/data</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details>0.887 e/A2</details>
        <segmentationList/>
        <micrographsFilePattern>data/20191216_gain-ref.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
