<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13121" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-10-27</depositionDate>
            <releaseDate>2025-12-15</releaseDate>
            <updateDate>2025-12-15</updateDate>
        </keyDates>
        <title>Cryo-EM movies of RNA bound human PNPase in loading state</title>
        <correspondingAuthor private="true">
            <authorORCID>0009-0007-5462-0984</authorORCID>
            <firstName>Ole</firstName>
            <lastName>Unseld</lastName>
            <organization type="academic">Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm</organization>
            <street>Solnavaegen 9</street>
            <townOrCity>Solna</townOrCity>
            <country>Sweden</country>
            <postOrZipCode>17165</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-6781-0345</authorORCID>
            <firstName>Martin</firstName>
            <lastName>Hallberg</lastName>
            <organization type="academic">Department of Cell and Molecular Biology, Karolinska Institutet</organization>
            <street>Solnavaegen 9</street>
            <townOrCity>Solna</townOrCity>
            <country>Sweden</country>
            <postOrZipCode>17165</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0009-0007-5462-0984">Unseld O</author>
            <author authorORCID="0000-0001-7495-7065">Das H</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">5.3</datasetSize>
        <entryDOI>10.6019/EMPIAR-13121</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-49590</emdbEntry>
            <emdbEntry>EMD-72352</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0009-0007-5462-0984" order="1">Unseld O</author>
                    <author authorORCID="0000-0001-7495-7065" order="2">Das H</author>
                    <author authorORCID="0000-0002-6781-0345" order="3">Hallberg BM</author>
                    <title>Loop-mediated regulation and base flipping drive RNA cleavage by human mitochondrial PNPase</title>
                    <journal>Nucleic Acid Research</journal>
                    <journalAbbreviation>NAR</journalAbbreviation>
                    <country>United Kingdom</country>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1093/nar/gkaf1296</externalReferences>
                    <details>Movie data, 0° (59.8 e/A^2 total dose) and 15°/20°/30°/35° (58.4 e/^2 total dose), Krios G3i, 300 kV, 2.7 mm spherical aberration, E-CFEG, -0.3 to -2.0 underfocus range, 0.5076 Å pixel size, BioQuantum-K3, coma-free alignments</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned movies of RNA bound human PNPase in loading state</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>20390</numImagesOrTiltSeries>
        <framesPerImage>59</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>59</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.5076</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.5076</pixelHeight>
        </dimensions>
        <details>Particle locations and stacks of the final reconstructions can be found in the EMBD-XXXXX folders. Different tilt movies have different frame numbers.</details>
        <segmentationList/>
        <micrographsFilePattern>data/</micrographsFilePattern>
        <pickedParticlesFilePattern>data/</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/</pickedParticlesDirectory>
    </imageSet>
</entry>
