Electron tomography of mitochondria from adult and aged mice hearts

REL 2025-09-24 2025-12-17 2026-01-05 Electron tomography of mitochondria from adult and aged mice hearts 0000-0002-9458-7150 Verónica Eisner Pontificia Universidad Católica de Chile Av. Libertador Bernardo O’Higgins 340 Santiago Región Metropolitana Chile 8331150 0000-0002-9458-7150 Verónica Eisner Pontificia Universidad Católica de Chile Santiago Chile 8331150 0000-0003-0525-0191 Karin Busch Institute of Integrative Cell Biology and Physiology, Faculty of Biology, University of Muenster Muenster Germany 48149 Barrientos G Molina-Riquelme I Breitsprecher L Busch KB Eisner V Agencia Nacional de Investigación y Desarrollo (ANID) 180060 Chile Bundesministerium für Bildung und Forschung (BMBF) 180060 Germany Agencia Nacional de Investigación y Desarrollo (ANID) 1231557 Chile 523.1 10.6019/EMPIAR-13004 ssET cell Molina-Riquelme I Barrientos G Breitsprecher L Gómez W Díaz-Castro F Morris S Almarza G Del Campo A Garrido-Olivares L Verdejo HE Psathaki OE Busch KB Eisner V Multiscale mitochondrial cristae remodeling links Opa1 downregulation to reduced OXPHOS capacity in aged hearts Proceedings of the National Academy of Sciences of the United States of America Proc Natl Acad Sci U S A 1 123 2025 English 10.1073/pnas.2508911123 41468425 Molina-Riquelme I Barrientos G Breitsprecher L Gómez W Díaz-Castro F Morris S del Campo A Garrido-Olivares L Verdejo H Psathaki OE Busch KB Eisner V Multi-scale mitochondrial cristae remodeling links Opa1 downregulation to reduced OXPHOS capacity in aged hearts bioRxiv 10.1101/2025.04.01.644555 Tilt-series of a cardiac mitochondrion from an adult mouse /data tilt series TIFF TIFF 129 1 UNSIGNED 16 BIT INTEGER 1024 20.4761 1024 20.4761

Tilt-series (±60°, 1° increment) were obtained in TALOS F200C G2 equipped with a Ceta 16M CMOS camera, with 28000X magnification and 200 kV. Stage drift was corrected in Inspect 3D software.

Tilt-series of a cardiac mitochondrion from an aged mouse /data tilt series TIFF TIFF 241 1 SIGNED 16 BIT INTEGER 512 40.9521 512 40.9521

Tilt-series (±60°, 0.5° increment) were obtained in TALOS F200C G2 equipped with a Ceta 16M CMOS camera, with 28000X magnification and 200 kV. Stage drift was corrected in Inspect 3D software.

Reconstructed volume of a cardiac mitochondrion from an adult mouse /data reconstructed volumes TIFF TIFF 63 1 SIGNED 16 BIT INTEGER 1024 20.4761 1024 20.4761

Volume was reconstructed using SIRT algorithm with 20 iterations in Inspect 3D software.

Reconstructed volume of a cardiac mitochondrion from an aged mouse /data reconstructed volumes TIFF TIFF 37 1 SIGNED 16 BIT INTEGER 512 40.9521 512 40.9521

Volume was reconstructed using SIRT algorithm with 20 iterations in Inspect 3D software.