REL 2025-09-09 2026-03-01 2026-03-01 0000-0003-3691-5010 Henriette Elisabeth Autzen University of Copenhagen Blegdamsvej 3B, 2200 Copenhagen N Copenhagen Denmark 2200 0000-0003-3691-5010 Henriette Elisabeth Autzen University of Copenhagen Blegdamsvej 3B, 2200 Copenhagen N Copenhagen Denmark 2200 Pugh CF Feilen LP Živković D Præstegaard KF Sideris C Borthwick NJ de Lichtenberg C Bolla JR Autzen AAA Autzen HE Novo Nordisk Foundation NNF20OC0060692 Denmark The Carlsberg Foundation CF20-0533 Denmark Danish Council for Independent Research 1131-00023 Denmark 3.8 10.6019/EMPIAR-13000 EMDB molecule EMD-55036 Pugh CF Feilen LP Živković D Præstegaard KF Sideris C Borthwick NJ de Lichtenberg C Bolla JR Autzen AAA Autzen HE Nature Communications Nat. Comms. Unaligned multi-frame micrographs of hTRPM4 in MSP2n" nanodiscs in Ca2+-bound state /data/20230428_TRPM4_CA_MSP_Grid1_krios-UCPH_raw micrographs - multiframe EER EER 7945 40 BIT 4096 0.725 4096 0.725

These are the EER files from Falcon 4i detector - raw micrographs, not motion corrected, acquired with EPU and a total dose of 52 e-/Å2 (1.3 e-/Å2 pr frame) Gain reference image can be found in the /data/ directory job028_particles.star contains all picked particles job034_partciles.star contains the subset of articles from 2D classification (first selection) job055_particles.star contains the subset of particles from the best classes after 3D classification that was further refined to the final reconstruction (second selection)

data/20230428_TRPM4_CA_MSP_Grid1_krios-UCPH_raw/GridSquare_*/Data/*_EER.eer data/Particle-stacks/job0*_particles.star data/Particle-stacks