REL 2025-09-17 2026-05-01 2026-05-01 0000-0003-3405-6021 Guillaume Gaullier Department of Chemistry - Ångström Laboratory Regementsvagen 10 Uppsala Sweden 752 37 0000-0001-5740-926X Cecilia Blikstad Department of Chemistry - Ångström Laboratory Regementsvagen 10 Uppsala Sweden 752 37 Gaullier G Vogiatzi N Blikstad C Swedish Research Council 2019-03700_VR Sweden Swedish Research Council for Sustainable Development 2019-01171_Formas Sweden Swedish Research Council 2023-05296_VR Sweden 1.3 10.6019/EMPIAR-12992 EMDB molecule EMD-54975 EMD-54976 EMD-54977 Vogiatzi N Gaullier G Blikstad C Raw movies /data/Images-Disc1 micrographs - multiframe TIFF TIFF 9745 40 UNSIGNED BYTE 5760 0.648 4092 0.648

The gain reference needs to be applied: gainref_counted.mrc (no flip nor rotation needed in CryoSPARC). Particle coordinates are provided as .cs files from CryoSPARC, also converted to .star files using pyem (see README file for details). J26_particles_exported: coordinates of hexamer particles J48_particles_exported: coordinates of dimer sub-particles (after C3 symmetry expansion and 3D classification), minor class J58_particles_exported: coordinates of dimer sub-particles (after C3 symmetry expansion and 3D classification), major class

data/Images-Disc1/GridSquare_*/Data/*_fractions.tiff data/*_particles_exported.star Gain reference file /data micrographs - single frame MRC MRC 1 1 32 BIT FLOAT 5760 variable 4092 variable

Gain reference file.

data/gainref_counted.mrc