REL 2025-09-09 2025-12-07 2025-12-07 Cryo-EM micrographs of PbAbiF dimer bound to ncRNA 0000-0003-4738-9503 Max Wilkinson Massachusetts Institute of Technology Cambridge Massachusetts United States 02142 0000-0003-0178-7995 Feng Zhang Massachusetts Institute of Technology Cambridge Massachusetts United States 02142 Wilkinson ME Zhang F 587.8 10.6019/EMPIAR-12962 EMDB molecule EMD-43682 Zilberzwige-Tal S Altae-Tran H Kannan S Wilkinson ME Vo SC Strebinger D Edmonds KK Yao CJ Mears KS Shmakov SA Makarova KS Macrae RK Koonin EV Zhang F Reprogrammable RNA-targeting CRISPR systems evolved from RNA toxin-antitoxins Cell Cell 7 188 1925 1940.e20 2025 English 10.1016/j.cell.2025.01.034 39970912 Unaligned multi-frame movies of PbAbiF RNP /data/Micrographs micrographs - multiframe TIFF TIFF 4272 40 UNSIGNED BYTE 5760 0.663 4092 0.663
Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM Voltage 300 keV Camera Gatan K3, super-resolution mode with 2-fold binning by EPU Energy filter GIF, 20eV slit width Magnification 130000 Pixel size 0.663 A/pix Dose 42.2 e/A2 Exposure rate 18.5 e/pix/s Exposure time 1.00 s Fractions 40 Total mics 4272 GainReference.mrc was binned using 'rebin by two' in Digital Micrograph. It needs to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can probably be used as is for cryoSPARC. particles.star contains metadata for the particle selected for the final reconstruction.
data/Micrographs/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff data/particles.star