REL 2025-08-28 2025-09-17 2025-09-17 0000-0001-6653-6682 Mark A Herzik Jr Department of Chemistry and Biochemistry, University of California San Diego 9500 Gilman Drive La Jolla California United States 92093 0000-0001-6653-6682 Mark A Herzik Jr Department of Chemistry and Biochemistry, University of California San Diego 9500 Gilman Drive La Jolla California United States 92093 Morizono MA Herzik Jr MA National Institutes of Health (NIH) R35-GM138206 United States National Institutes of Health (NIH) T32-GM008326 United States Kinship Foundation Searle Scholars Program United States 4.7 10.6019/EMPIAR-12953 EMDB molecule EMD-44675 Morizono MA McGuire KL Birouty NI Herzik MA Nature communications Nat Commun 1 15 10815 10815 2024 English 10.1038/s41467-024-54499-1 39737924

CryoEM movies of the human mortalin(R126W)-GrpEL1(WT) complex. Data were collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 130,000x in EF-TEM mode (with a calibrated pixel size of 0.935 Å) on a Falcon 4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2 with a defocus range of − 1.0 to − 2.5 µm. Data were collected automatically using EPU with aberration free image shift.

Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex - dataset 1 /data/Movies_1 micrographs - multiframe EER EER 952 2898 32 BIT FLOAT 4096 0.935 4096 0.935

Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 130,000x in EF-TEM mode (with a calibrated pixel size of 0.935 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. Gain file required for motion correction of unaligned micrographs for dataset 1 is included.

Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex - dataset 2 /data/Movies_2 micrographs - multiframe EER EER 1757 2898 32 BIT FLOAT 4096 0.935 4096 0.935

Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 130,000x in EF-TEM mode (with a calibrated pixel size of 0.935 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. Gain file required for motion correction of unaligned micrographs for dataset 2 is included.

Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex - dataset 3 /data/Movies_3 micrographs - multiframe EER EER 1861 2044 32 BIT FLOAT 4096 0.935 4096 0.935

Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 130,000x in EF-TEM mode (with a calibrated pixel size of 0.935 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. Gain file required for motion correction of unaligned micrographs for dataset 3 is included.