<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12925" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-08-12</depositionDate>
            <releaseDate>2025-12-08</releaseDate>
            <updateDate>2025-12-08</updateDate>
        </keyDates>
        <title>Complex between human carboxyhemoglobin and Staphylococcus aureus hemophore IsdH</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-3916-8858</authorORCID>
            <firstName>Omar</firstName>
            <lastName>De Bei</lastName>
            <organization type="academic">Department of Medicine and Surgery, University of Parma</organization>
            <street>Via Volturno, 39</street>
            <townOrCity>Parma</townOrCity>
            <stateOrProvince>Parma</stateOrProvince>
            <country>Italy</country>
            <postOrZipCode>43125</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6787-0594</authorORCID>
            <firstName>Stefano</firstName>
            <lastName>Bettati</lastName>
            <organization type="academic">Department of Medicine and Surgery, University of Parma</organization>
            <street>Via Volturno, 39</street>
            <townOrCity>Parma</townOrCity>
            <stateOrProvince>Parma</stateOrProvince>
            <country>Italy</country>
            <postOrZipCode>43125</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-3115-2122">Buoli Comani V</author>
            <author authorORCID="0000-0003-3916-8858">De Bei O</author>
            <author authorORCID="0000-0002-3097-1743">Gragera M</author>
            <author authorORCID="0000-0003-1144-9877">Luisi BF</author>
            <author authorORCID="0000-0001-6787-0594">Bettati S</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Ministero dell Universita e della Ricerca</fundingBody>
                <code>2020AE3LTA</code>
                <country>Italy</country>
            </grantReference>
            <grantReference>
                <fundingBody>Wellcome Trust</fundingBody>
                <code>200873/Z/16/Z</code>
                <country>United Kingdom</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-12925</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-54546</emdbEntry>
            <emdbEntry>EMD-54565</emdbEntry>
            <emdbEntry>EMD-54566</emdbEntry>
            <emdbEntry>EMD-54567</emdbEntry>
            <emdbEntry>EMD-54563</emdbEntry>
            <emdbEntry>EMD-54619</emdbEntry>
            <emdbEntry>EMD-54618</emdbEntry>
            <emdbEntry>EMD-54620</emdbEntry>
            <emdbEntry>EMD-54621</emdbEntry>
            <emdbEntry>EMD-54613</emdbEntry>
            <emdbEntry>EMD-54591</emdbEntry>
            <emdbEntry>EMD-54585</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0003-1144-9877" order="1">Buoli Comani V</author>
                    <author authorORCID="0000-0003-3916-8858" order="2">De Bei O</author>
                    <author authorORCID="0009-0005-9013-0283" order="3">Pancrazi F</author>
                    <author authorORCID="0000-0002-3097-1743" order="4">Gragera M</author>
                    <author authorORCID="0000-0002-5610-2722" order="5">Paris G</author>
                    <author authorORCID="0000-0002-4615-6786" order="6">Marchetti M</author>
                    <author authorORCID="0000-0003-0359-0627" order="7">Campanini B</author>
                    <author authorORCID="0000-0002-5389-2669" order="8">Ronda L</author>
                    <author authorORCID="0000-0002-7447-9945" order="9">Faggiano S</author>
                    <author authorORCID="0000-0003-3298-6639" order="10">Bizzarri AR</author>
                    <author authorORCID="0000-0001-6787-0594" order="11">Bettati S</author>
                    <title>Hemoglobin Receptor Redundancy in Staphylococcus aureus: Molecular Flexibility as a Determinant of Divergent Hemophore Activity</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of human carboxyhemoglobin bound to Staphylococcus aureus IsdH-N2N3</name>
        <directory>/data/micrographs</directory>
        <category>micrographs - focal pairs - unprocessed</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>11114</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.645</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.645</pixelHeight>
        </dimensions>
        <details>The analysed protein–protein complex exhibits substantial heterogeneity. Five distinct particle subsets were identified to describe this heterogeneity, and each subset has been deposited as a separate particle stack.</details>
        <segmentationList/>
        <micrographsFilePattern>data/micrographs/Movie*/FoilHole_*_Data_*_*_*_*_fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern>data/particles_*_complex/*_run_data_GLOBUS.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/particles_*_complex/Movie*</pickedParticlesDirectory>
    </imageSet>
</entry>
