REL
2025-07-30
2026-03-04
2026-04-07
CryoEM dataset of M. mazei topoisomerase VI(A:E342Q)-minicircle DNA complex
0000-0002-3602-5651
Daniel
Richman
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
725 N Wolfe St
Baltimore
MD
United States
21205
0000-0003-0666-1240
James
Berger
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
725 N Wolfe St
Baltimore
MD
United States
21205
Richman DE
Berger JM
National Institutes of Health/National Cancer Institute (NIH/NCI)
5R35 CA263778
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
1F32 GM128269
United States
14.1
10.6019/EMPIAR-12914
EMDB
molecule
EMD-49972
EMD-70206
Richman DE
Wendorff TJ
Rashid F
Beck C
Yan Q
Johnson HR
Eckerty RA
Fogg JM
Baker ML
Zechiedrich L
Berger JM
Supercoiled DNA recognition and cleavage control in topoisomerase VI
Nature communications
Nat Commun
1
17
2026
English
10.1038/s41467-026-69491-0
41698922
These micrographs are unaligned eer files from one microscopy session and grid.
Unaligned eer micrographs of M mazei Top6(A:E342Q)-306 bp minicircle DNA-ADPNP complex
/data
micrographs - multiframe
EER
EER
26728
1233
BIT
4096
0.93
4096
0.93
This dataset was collected with no stage tilt. Data were collected at the Beckman Center for CryoEM at Johns Hopkins University School of Medicine on a Krios G3i, camera: TFS Falcon 4i, image shift at 130kx mag, exposure settings: ~4.0 s [images saved in eer format], e flux: ~8 e-/px/s with ~40 e-/Å^2 total dose, pixel size: ~0.93 Å/px (e- counting with an image dimension of 4096x4096 px), energy filter: Selectris 10 eV, defocus range: -0.8 µm to -1.8 µm (nominal).