Cryo-electron microscopy of the bacterial phytochrome PaBphP in its PrPfr intermediate state

REL 2025-06-18 2025-09-04 2025-09-04 Cryo-electron microscopy of the bacterial phytochrome PaBphP in its PrPfr intermediate state 0000-0002-6961-8015 Sebastian Westenhoff Uppsala University Uppsala Sweden 752 37 0000-0002-6961-8015 Sebastian Westenhoff Uppsala University Uppsala Sweden 752 37 Bodizs S Westenhoff S 13.2 10.6019/EMPIAR-12874 EMDB molecule EMD-53916 Bodizs SA Fischer AM Cervenak M Prodhan S Maj M Westenhoff S Chemical mechanism of allosteric and asymmetric dark reversion in a bacterial phytochrome uncovered by cryo-EM bioRxiv 2025 10.1101/2025.08.21.671592 Raw micrographs of PaBphP 120 s into dark reversion /data/PaBphP_darkreversion_120s_micrographs micrographs - multiframe TIFF TIFF 13534 50 0 49 UNSIGNED BYTE 4092 0.828 5760 0.828

data/PaBphP_darkreversion_120s_micrographs/k3_frames_a/Images-Disc1/*/Data/*.tiff Raw micrographs of PaBphP 300 s into dark reversion /data/PaBphP_darkreversion_300s_micrographs micrographs - multiframe TIFF TIFF 14573 50 0 49 UNSIGNED BYTE 4092 0.828 5760 0.828

data/PaBphP_darkreversion_300s_micrographs/k3_frames_a/Images-Disc1/*/Data/*.tiff Raw micrographs of PaBphP 960 s into dark reversion /data/PaBphP_darkreversion_960s_micrographs micrographs - multiframe TIFF TIFF 13112 50 0 49 UNSIGNED BYTE 4092 0.828 5760 0.828

data/PaBphP_darkreversion_960s_micrographs/k3_frames_b/Images-Disc1/*/Data/*.tiff Aligned particles for the PrPr form of PaBphP 120 s into dark reversion /data/PaBphP_darkreversion_120s_PrPr_particles picked particles - single frame - processed MRC MRC 363194 1 UNSIGNED BYTE 400 0.828 400 0.828

data/PaBphP_darkreversion_120s_PrPr_particles/J949_split_0/J910/restack/*.mrc data/PaBphP_darkreversion_120s_PrPr_particles/J949_split_0/J910/restack Aligned particles for the PrPfr form of PaBphP 120 s into dark reversion /data/PaBphP_darkreversion_120s_PrPfr_particles picked particles - single frame - processed MRC MRC 527123 1 UNSIGNED BYTE 400 0.828 400 0.828

data/PaBphP_darkreversion_120s_PrPfr_particles/J909_split_0/J908/restack/*.mrc data/PaBphP_darkreversion_120s_PrPfr_particles/J909_split_0/J908/restack/ Aligned particles for the PrPfr form of PaBphP 300 s into dark reversion /data/PaBphP_darkreversion_300s_PrPfr_particles picked particles - single frame - processed MRC MRC 964467 1 UNSIGNED BYTE 400 0.828 400 0.828

data/PaBphP_darkreversion_300s_PrPfr_particles/J913_split_0/J912/restack/*.mrc data/PaBphP_darkreversion_300s_PrPfr_particles/J913_split_0/J912/restack/ Aligned particles for the PrPfr form of PaBphP 960 s into dark reversion /data/PaBphP_darkreversion_960s_PrPfr_particles picked particles - single frame - processed MRC MRC 303375 1 UNSIGNED BYTE 400 0.828 400 0.828

data/PaBphP_darkreversion_960s_PrPfr_particles/J947_split_0/J946/restack/batch_0_restacked.mrc data/PaBphP_darkreversion_960s_PrPfr_particles/J947_split_0/J946/restack/ Aligned particles for the PfrPfr form of PaBphP 960 s into dark reversion /data/PaBphP_darkreversion_960s_PfrPfr_particles picked particles - single frame - processed MRC MRC 172997 1 UNSIGNED BYTE 400 0.828 400 0.828

data/PaBphP_darkreversion_960s_PfrPfr_particles/J945_split_0/J944/restack/batch_0_restacked.mrc data/PaBphP_darkreversion_960s_PfrPfr_particles/J945_split_0/J944/restack/