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        <keyDates>
            <depositionDate>2025-06-12</depositionDate>
            <releaseDate>2026-06-16</releaseDate>
            <updateDate>2026-06-16</updateDate>
        </keyDates>
        <title>Single particle cryo-EM of Staphylococcus aureus YhaM D193A hexamers in complex with single-stranded substrate RNA</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-8821-688X</authorORCID>
            <firstName>Christine</firstName>
            <middleName>M</middleName>
            <lastName>Dunham</lastName>
            <organization type="academic">Department of Chemistry, Emory University</organization>
            <street>1515 Dickey Dr Room 455</street>
            <townOrCity>Atlanta</townOrCity>
            <stateOrProvince>Georgia</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>30322</postOrZipCode>
        </correspondingAuthor>
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            <firstName>Christine</firstName>
            <middleName>M</middleName>
            <lastName>Dunham</lastName>
            <organization type="academic">Department of Chemistry, Emory University</organization>
            <street>1515 Dickey Dr Room 455</street>
            <townOrCity>Atlanta</townOrCity>
            <stateOrProvince>Georgia</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>30324</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-0202-915X">Mattingly JM</author>
            <author authorORCID="0000-0003-4148-7364">Tanquary JR</author>
            <author authorORCID="0000-0002-8821-688X">Dunham CM</author>
        </authorsList>
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                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R01GM121359</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</fundingBody>
                <code>R01AI150986</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R01GM093278</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>T32GM8367</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
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        <entryDOI>10.6019/EMPIAR-12851</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-70208</emdbEntry>
            <emdbEntry>EMD-70886</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0003-0202-915X" order="1">Mattingly JM</author>
                    <author authorORCID="0000-0003-0287-7802" order="2">Liponska A</author>
                    <author authorORCID="0000-0003-4148-7364" order="3">Tanquary JT</author>
                    <author authorORCID="0000-0003-4213-4050" order="4">Yap MNF</author>
                    <author authorORCID="0000-0002-8821-688X" order="5">Dunham CM</author>
                    <title>Single particle cryo-EM of Staphylococcus aureus YhaM D193A hexamers in complex with single-stranded substrate RNA</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                    <details>Micrographs are unaligned 40-frame movies with a nominal accumulated dose of 47.04 electrons per square angstrom. Images were collected using a Gatan K3 direct electron detector operating in super-resolution mode with a pixel size of 0.4125 angstroms per pixel.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of Staphylococcus aureus YhaM D193A hexamers bound to single-stranded substrate RNA</name>
        <directory>/data/rawdata</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>14007</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>0</frameRangeMin>
            <frameRangeMax>39</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.4125</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.4125</pixelHeight>
        </dimensions>
        <details>Micrographs are provided as multi-frame movies collected in super-resolution mode on a Gatan K3 direct electron detector and have filenames terminating in "frames.tif". The "references" subdirectory contains an MRC-format gain-reference file. The "xml" subdirectory contains Thermo EPU-format XML metadata files with image shift information which can be used for image shift group splitting.</details>
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        <micrographsFilePattern></micrographsFilePattern>
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        <pickedParticlesDirectory></pickedParticlesDirectory>
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