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        <keyDates>
            <depositionDate>2025-06-05</depositionDate>
            <releaseDate>2025-10-27</releaseDate>
            <updateDate>2025-10-27</updateDate>
        </keyDates>
        <title>E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418)</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-2419-380X</authorORCID>
            <firstName>Graeme</firstName>
            <middleName>L</middleName>
            <lastName>Conn</lastName>
            <organization type="academic">Department of Biochemistry, Emory University</organization>
            <street>1510 Clifton Road NE Room 4031</street>
            <townOrCity>Atlanta</townOrCity>
            <stateOrProvince>Georgia</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>30322</postOrZipCode>
        </correspondingAuthor>
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            <firstName>Graeme</firstName>
            <middleName>L</middleName>
            <lastName>Conn</lastName>
            <organization type="academic">Department of Biochemistry, Emory University</organization>
            <street>1510 Clifton Road NE Room 4031</street>
            <townOrCity>Atlanta</townOrCity>
            <stateOrProvince>Georgia</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>30322</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-8821-688X</authorORCID>
            <firstName>Christine</firstName>
            <middleName>M</middleName>
            <lastName>Dunham</lastName>
            <organization type="academic">Department of Chemistry, Emory University</organization>
            <street>1515 Dickey Dr Room 455</street>
            <townOrCity>Atlanta</townOrCity>
            <stateOrProvince>Georgia</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>30322</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-0202-915X">Mattingly JM</author>
            <author authorORCID="0000-0002-0222-6092">Dey D</author>
            <author authorORCID="0000-0002-9387-051X">Zelinskaya N</author>
            <author authorORCID="0000-0002-8821-688X">Dunham CM</author>
            <author authorORCID="0000-0002-2419-380X">Conn GL</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</fundingBody>
                <code>R01AI088025</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>T32GM135060</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>U24GM129547</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>U24GM129539</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Simons Foundation</fundingBody>
                <code>SF349247</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Center for Advancing Translational Sciences (NIH/NCATS)</fundingBody>
                <code>UL1TR000454</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
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        <entryDOI>10.6019/EMPIAR-12844</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-44194</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0003-0202-915X" order="1">Mattingly JM</author>
                    <author authorORCID="0000-0002-0222-6092" order="2">Dey D</author>
                    <author authorORCID="0000-0002-9387-051X" order="3">Zelinskaya N</author>
                    <author authorORCID="0000-0002-8821-688X" order="4">Dunham CM</author>
                    <author authorORCID="0000-0002-2419-380X" order="5">Conn GL</author>
                    <title>Basis for selective drug evasion of an aminoglycoside-resistance ribosomal RNA modification</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                    <details>Raw multi-frame micrographs were collected with an accumulated dose of 50 electrons per square angstrom.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of E. coli 70S ribosome complexes containing P-site tRNA-fMet, A-site tRNA-Val, N1-methylated 16S nucleotide A1408, and G418</name>
        <directory>/data/frames</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>5302</numImagesOrTiltSeries>
        <framesPerImage>29</framesPerImage>
        <frameRange>
            <frameRangeMin>0</frameRangeMin>
            <frameRangeMax>28</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.814</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.814</pixelHeight>
        </dimensions>
        <details>Micrographs are gain-corrected multi-frame TIFF movies collected using a Thermo Fisher Titan Krios operating at 300 kV and a Thermo Fisher Falcon 4i direct electron detector. Images were collected with a total accumulated dose of 50 electrons per square angstrom. Image filenames terminate in "Fractions.tif". Files whose names terminate in "Fractions.xml" contain EPU-format image shift data that can be used for image shift group splitting.</details>
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        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
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