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        <keyDates>
            <depositionDate>2025-05-22</depositionDate>
            <releaseDate>2026-01-15</releaseDate>
            <updateDate>2026-01-15</updateDate>
        </keyDates>
        <title>Cryo-electron microscopy of virus-like particle composed of PVY coat protein with Q87H mutation</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-8045-7224</authorORCID>
            <firstName>Neža</firstName>
            <lastName>Koritnik</lastName>
            <organization type="academic">Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry</organization>
            <townOrCity>Ljubljana</townOrCity>
            <country>Slovenia</country>
            <postOrZipCode>1000</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6786-9737</authorORCID>
            <firstName>Marjetka</firstName>
            <lastName>Podobnik</lastName>
            <organization type="academic">Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry</organization>
            <townOrCity>Ljubljana</townOrCity>
            <country>Slovenia</country>
            <postOrZipCode>1000</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-8045-7224">Koritnik N</author>
            <author authorORCID="0000-0003-2407-9297">Kežar A</author>
            <author authorORCID="0000-0001-6786-9737">Podobnik M</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Slovenian Research Agency</fundingBody>
                <code>J1-4410</code>
                <country>Slovenia</country>
            </grantReference>
            <grantReference>
                <fundingBody>Slovenian Research Agency</fundingBody>
                <code>P1-0391</code>
                <country>Slovenia</country>
            </grantReference>
            <grantReference>
                <fundingBody>Slovenian Research Agency</fundingBody>
                <code>IO-0003</code>
                <country>Slovenia</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">1021.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-12830</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>virus</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-53638</emdbEntry>
            <emdbEntry>EMD-53639</emdbEntry>
            <emdbEntry>EMD-53640</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
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                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0002-8045-7224" order="1">Koritnik N</author>
                    <author authorORCID="0000-0003-2407-9297" order="2">Kežar A</author>
                    <author authorORCID="0000-0002-2665-3582" order="3">Kavčič L</author>
                    <author authorORCID="0000-0002-4349-3752" order="4">Tušek Žnidarič M</author>
                    <author authorORCID="0000-0001-9854-9955" order="5">Leonardi A</author>
                    <author authorORCID="0000-0002-3044-5989" order="6">Pollari M</author>
                    <author authorORCID="0000-0001-8233-8942" order="7">Mäkinen K</author>
                    <author authorORCID="0000-0001-6786-9737" order="8">Podobnik M</author>
                    <title>Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particles</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <externalReferences type="doi">10.1038/s42003-025-09502-w</externalReferences>
                </journalCitation>
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        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multiframe micrographs of PVY VLP with CP mutation Q87H</name>
        <directory>/data/Movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1353</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.95</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.95</pixelHeight>
        </dimensions>
        <details>The movies were imaged (Glacios, 200 kV) in counting mode with Falcon3 (Thermo Fisher Scientific) direct electron detector at a nominal magnification of 150,000 with a total electron dose of 40 e-/Å2.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
