REL 2025-04-25 2025-10-09 2025-10-09 0000-0002-4461-750X Jenny Sachweh Department of Molecular Sociology, Max-Planck-Institute of Biophysics Max-von-Laue-Straße 3 Frankfurt Hesse Germany 60438 0000-0002-7397-1321 Martin Beck Department of Molecular Sociology, Max-Planck-Institute of Biophysics Max-von-Laue-Straße 3 Frankfurt Hesse Germany 60438 Sachweh J Beck M Hessian Ministry of Science, Higher Education and Art (HMWK) EnABLE Germany European Research Council (ERC) 724379-Complex Assembly Germany European Research Council (ERC) 101054823-NPCvalve Germany 176.6 10.6019/EMPIAR-12780 EMDB cell EMD-53721 EMD-53722 EMD-53723 EMD-53724 EMD-53725 EMD-53727 EMD-53728 EMD-53729 EMD-53731 EMD-53732 Sachweh J Börmel M Klumpe S Becker A Taniguchi R Kubańska MA Pintschovius V Kaindl E Plitzko JM Wilfling F Beck M Hampoelz B Cell Cell 2025 English 10.1016/j.cell.2025.07.025 40829587 Tilt series of S. cerevisiae Not4Δ Nup188::GFP Nup170::mars cells /data/EMPIAR_Yeast/raw_data tilt series MRC MRC 40 61 42 61 SIGNED 16 BIT INTEGER 5760 2.176 4092 2.176

Tilt series were frame-aligned in SerialEM during acquisition, mrc and mdoc files for the stacks are provided. The subfolder "alignment_files", contains alignment files (.xf) and tilt files (.tlt) that were generated by AreTomo, CTF estimation from gctf are included as gctf.star files. The cleaned tilt fils lists images excluded from raw stacks.

Filtered tomograms of S. cerevisiae Not4Δ Nup188::GFP Nup170::mars cells /data/EMPIAR_Yeast/tomograms reconstructed volumes MRC MRC 40 475 200 475 32 BIT FLOAT 1016 8.704 1440 8.704

Non-CTF corrected tomograms reconstructed at bin 4 with AreTomo and processed by SIRT-like filtering in IMOD are provided.