REL
2025-05-14
2025-09-04
2025-09-04
Cryo-ET of D. melanogaster Nup358::VN/Nup358::VC cells
0000-0002-4461-750X
Jenny
Sachweh
Department of Molecular Sociology, Max-Planck-Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt
Hesse
Germany
60438
0000-0002-7397-1321
Martin
Beck
Department of Molecular Sociology, Max-Planck-Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt
Hesse
Germany
60438
Sachweh J
Beck M
Hessian Ministry of Science, Higher Education and Art (HMWK)
EnABLE
Germany
European Research Council (ERC)
724379-Complex Assembly
Germany
European Research Council (ERC)
101054823-NPCvalve
Germany
307.9
10.6019/EMPIAR-12779
EMDB
cell
EMD-53739
EMD-53740
EMD-53741
EMD-53742
EMD-53743
EMD-53744
EMD-53745
EMD-53746
EMD-53747
EMD-53750
EMD-53751
Sachweh J
Börmel M
Klumpe S
Becker A
Taniguchi R
Kubańska MA
Pintschovius V
Kaindl E
Plitzko JM
Wilfling F
Beck M
Hampoelz B
The small GTPase Ran defines Nuclear Pore Complex Asymmetry
Tilt series of D. melanogaster Nup358::VN/Nup358::VC cells
/data/EMPIAR_Fly/raw_data
tilt series
MRC
MRC
111
61
42
61
SIGNED 16 BIT INTEGER
4096
2.414
4096
2.414
Tilt series were frame-aligned in SerialEM during acquisition, mrc and mdoc files for the stacks are provided. The subfolder "alignment_files", contains alignment files (.xf) and tilt files (.tlt) that were generated by IMOD, CTF estimation from gctf are included as gctf.star files. The cleaned tilt fils lists images excluded from raw stacks.
Filtered tomograms of D. melanogaster Nup358::VN/Nup358::VC cells
/data/EMPIAR_Fly/tomograms
reconstructed volumes
MRC
MRC
111
500
SIGNED 16 BIT INTEGER
1024
9.656
1024
9.656
Non-CTF corrected tomograms reconstructed at bin 4 and processed by SIRT-like filtering in IMOD are provided.