REL
2025-04-04
2025-05-14
2025-05-14
Single-particle cryo-EM of Staphylococcus aureus ribosomes inhibited by fusidic acid and fusidic acid cyclopentane
0000-0002-4302-6855
Adrián
González-López
Department of Cell and Molecular Biology (ICM), Uppsala University
Box 596
Uppsala
Uppland
Sweden
75124
0000-0001-9079-2774
Maria
Selmer
Department of Cell and Molecular Biology (ICM), Uppsala University
Box 596
Uppsala
Uppland
Sweden
75124
González-López A
Larsson DSD
Selmer M
Swedish Research Council
2017-03827
Sweden
Swedish Research Council
2022-04511
Sweden
11.1
10.6019/EMPIAR-12723
EMDB
molecule
EMD-17365
EMD-17363
EMD-17364
González-López A
Larsson DSD
Koripella RK
Cain BN
Chavez MG
Hergenrother PJ
Sanyal S
Selmer M
Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane
Scientific reports
Sci Rep
1
14
2024
English
10.1038/s41598-024-64868-x
38902339
Unaligned multi-frame micrographs of FA-CP inhibited ribosomes
/data/FA-CP_Krios_dataset/raw_movies
micrographs - multiframe
EER
EER
17252
684
UNSIGNED BYTE
4096
0.728
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Data (raw movies) were collected at SciLifeLab in Umeå, Sweden in a Titan Krios G2 operated at 300 kV and equipped with a Falcon-4i direct electron detector and Selectris energy filter using 10 eV slit. The data were acquired at 165,000 x nominal magnification with a calibrated pixel size of 0.728 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 520 nm beam size. The EER formatted movies were collected in 684 raw frames with a total dose of 27.77 e-/Å2 (12.45 e-/pixel/s) over 2.23 s with a set defocus between -0.7 to -1.3 µm.
The movies are not gain-corrected, use the file provided within the main folder.
Unaligned multi-frame micrographs of FA inhibited ribosomes
/data/FA_Krios_dataset/raw_movies
micrographs - multiframe
TIFF
TIFF
5486
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UNSIGNED BYTE
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Data (raw movies) were collected at SciLifeLab in Solna, Sweden on a Titan Krios G2 operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at 105,000 x nominal magnification with a calibrated pixel size of 0.824 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 670 nm beam size. The movies were collected in 30 frames over 1.3 s with a total dose of 30.83 e-/Å2 (16.1 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.
The movies are gain-corrected.
EMD-17364 particles (FA-CP CHI)
/data/FA-CP_Krios_dataset/particles_EMD-17364_PDB-8P2G
picked particles - single frame - processed
MRC
MRC
303005
1
UNSIGNED BYTE
700
0.728
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0.728
Raw movies from the FA-CP inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 31 frames over 28.74 s with a total dose of 29.8 e-/Å2 (0.94 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.
The movies are gain corrected.
Unaligned multi-frame micrographs of FA inhibited ribosomes screening Glacios data
/data/FA_Glacios_screening_data/raw_movies
micrographs - multiframe
TIFF
TIFF
1460
30
SIGNED 16 BIT INTEGER
4096
0.952
4096
0.952
Raw movies from the FA inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 30 frames over 27.79 s with a total dose of 30 e-/Å2 (1.00 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.
The movies are gain corrected.