<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12722" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-04-22</depositionDate>
            <releaseDate>2025-05-30</releaseDate>
            <updateDate>2025-05-30</updateDate>
        </keyDates>
        <title>Structural insights into transcriptional regulation by the helicase RECQL5</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9816-3681</authorORCID>
            <firstName>Eva</firstName>
            <lastName>Nogales</lastName>
            <organization type="academic">University of California Berkeley</organization>
            <street>University Dr</street>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94706</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9816-3681</authorORCID>
            <firstName>Eva</firstName>
            <lastName>Nogales</lastName>
            <organization type="academic">University of California Berkeley</organization>
            <street>University Dr</street>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94704</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-2429-4659">Florez Ariza AJ</author>
            <author authorORCID="0000-0002-4236-9127">Lue NZ</author>
            <author authorORCID="0000-0002-8330-8961">Grob P</author>
            <author authorORCID="0000-0002-6569-3608">Kaeser B</author>
            <author authorORCID="0009-0008-3181-3799">Fang J</author>
            <author authorORCID="0000-0003-4322-5886">Kassube SA</author>
            <author authorORCID="0000-0001-9816-3681">Nogales E</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Howard Hughes Medical Institute (HHMI)</fundingBody>
                <code>none</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">2.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-12722</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-48076</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0003-2429-4659" order="1">Florez Ariza AJ</author>
                    <author authorORCID="0000-0002-4236-9127" order="2">Lue NZ</author>
                    <author order="3">Grob P</author>
                    <author order="4">Kaeser B</author>
                    <author order="5">Fang J</author>
                    <author order="6">Kassube SA</author>
                    <author authorORCID="0000-0001-9816-3681" order="7">Nogales E</author>
                    <title>Structural insights into transcriptional regulation by the helicase RECQL5</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2025</year>
                    <externalReferences type="doi">10.1101/2025.01.29.634372</externalReferences>
                    <externalReferences type="pubmed">39975028</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of human RNA Polymerase II elongation complex bound to the RECQL5 helicase in the presence of ADP</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>9048</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.048</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.048</pixelHeight>
        </dimensions>
        <details>This dataset contains raw, uncorrected movies collected in non-CDS, non-super-resolution mode (collected using a Titan Krios G3i, 300 kV, spherical aberration 2.7 mm, total dose 50 e/A^2). The pixel size is 1.048 A. The directory also contains the gain reference file (CountRef_240917_RECQL5-ADP_176-1_0001_X-2Y+0-1.mrc). This should not be rotated or flipped for processing in cryoSPARC.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
