REL 2025-04-08 2025-04-23 2025-04-23 0000-0003-4738-9503 Max Wilkinson Massachusetts Institute of Technology 75 Ames Street Cambridge Massachusetts United States 02142 0000-0003-0178-7995 Feng Zhang Broad Institute of Harvard and MIT 75 Ames Street Cambridge Massachusetts United States 02142 Faure G Saito M Wilkinson ME Quinones-Olvera N Xu P Flam-Shepherd D Kim S Reddy N Zhu S Evgeniou L Koonin EV Macrae RK Zhang F 1.9 10.6019/EMPIAR-12705 EMDB molecule EMD-48616 Faure G Saito M Wilkinson ME Quinones-Olvera N Xu P Flam-Shepherd D Kim S Reddy N Zhu S Evgeniou L Koonin EV Macrae RK Zhang F Science (New York, N.Y.) Science United States 2025 English 10.1126/science.adv9789 40014690 Unaligned multi-frame movies of the TIGR-TasR RNP bound to target DNA /data/micrographs micrographs - multiframe TIFF TIFF 14257 40 UNSIGNED BYTE 5760 0.663 4092 0.663

Unaligned, non-gain-normalised, 40-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning (within EPU) at 130,000x magnification on the MIT.nano Titan Krios G3i microscope operated at 300 keV (Cs = 2.7mm). Each micrograph accumulates 45.4 e/A2 over a 1.09s exposure time. The final refinement run_data.star file for the EMDB deposition is included as 'EMD-46818_refine_data.star', although you will need to edit the micrograph path within this file if you wish to reuse the picked coordinates. Good luck! This dataset is a little bit tricky.

data/micrographs/GridSquare_*/Data/FoilHole_*_Data_*_fractions.tiff data/EMD-48616_refine_data.star