REL 2025-03-16 2025-05-22 2025-05-22 0000-0002-4343-8358 Johan Decelle Photosymbiosis, PCV, CNRS-CEA 17 rue des Martyrs Grenoble Isere France 38054 0000-0002-5337-2209 Ananya Kedige Rao Photosymbiosis, PCV, CNRS-CEA 17 rue des Martyrs Grenoble France 38054 Rao AK Gallet B Jouneau PH Decelle J Agence Nationale de la Recherche (ANR) ANR-21-CE02-0030 France Agence Nationale de la Recherche (ANR) ANR-22-PEEL-0014 France European Research Council (ERC) 101088661 France Gordon and Betty Moore Foundation GBMF11532 United States 272.6 10.6019/EMPIAR-12627 FIB-SEM cell Rao AK Yee D Chevalier F Seifi M Jug F Deschamps J Wu TD Gast R Jouneau PH Decelle J Olivetta M Dudin O Gallet B Hehenberger E bioRxiv bioRxiv France 2024 English 10.1101/2024.10.20.619283 Rao AK Yee D Chevalier F LeKieffre C Pavie M Olivetta M Dudin O Gallet B Hehenberger E Seifi M Jug F Deschamps J Wu TD Gast R Jouneau PH Decelle J Current biology : CB Curr Biol 2025 English 10.1016/j.cub.2025.03.076 40250433 Aligned Fib-SEM image stacks of 3 different continuously fed RSD Host cells /data/Host-RSD/Host-RSD-Cont.Fed micrographs - multiframe TIFF TIFF 3598 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

Pixel size for Cell 1 is 6nm with a total of 3598 frames/slices. Cell 2 and Cell 3 are caputred at 5nm resolution with 3280 and 2659 frames respectively. All stacks were aligned and cropped. For segmentation, voxel type was reduced to 8 bits and further binned if necessary (Indicated in supplementary table S3 of the article)

Aligned Fib-SEM image stacks of 3 different RSD Host cells starved for 9 weeks /data/Host-RSD/Host-RSD-Starved9week micrographs - multiframe TIFF TIFF 2244 1 BIT variable variable variable variable

Pixel size for Cell 1, 2 and 3 is 8nm with 695, 1874 and 2244 frames respectively. All stacks were aligned, cropped and converted from 16 bit to 8 bit voxel type. Cell 1 and 3 are inverted. Any binning done during segmentation are indicated in supplementary table S3 of the article

Aligned Fib-SEM image stacks of 5 different prey /Phaeocystis antarctica/ cells in possibly dividing stages /data/Prey-Phaant/Prey-Phaant-Divding micrographs - multiframe TIFF TIFF 755 1 BIT variable variable variable variable

All cells were captured with a 6nm resolution with about 660-755 frames. All stacks were aligned and cropped. Cells 4 and 5 are inverted and converted to 8 bit voxel type while cells 1-3 remain as 16 bit voxel type. For segmentation, voxel type for all cells was reduced to 8 bits

Aligned Fib-SEM image stacks of 7 different prey /Phaeocystis antarctica/ cells /data/Prey-Phaant/Prey-Phaant-NonDividing micrographs - multiframe TIFF TIFF 688 1 BIT variable variable variable variable

All cells were captured with a 6nm resolution with about 462-688 frames. All stacks were aligned and cropped. Cells 4-7 are inverted and converted to 8 bit voxel type while cells 1-3 remain as 16 bit voxel type. For segmentation, voxel type was reduced to 8 bits for all cells