REL 2025-03-06 2025-04-07 2025-04-07 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 0000-0002-1332-128X Elizabeth A Campbell The Rockefeller University 1230 York Avenue New York NY United States 10065 Malone BF Perry JK Olinares PDB Lee HW Chen J Appleby TC Feng JY Bilello JP Ng H Sotiris J Ebrahim M Chua EYD Mendez JH Eng ET Landick R Götte M Chait BT Campbell EA Darst SA Simons Foundation SF349247 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) P41` GM103310 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) U24 GM129539 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) P41 GM103314 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) P41 GM109824 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01 AI61278 United States 2.8 10.6019/EMPIAR-12607 EMDB molecule EMD-26641 Malone BF Perry JK Olinares PDB Lee HW Chen J Appleby TC Feng JY Bilello JP Ng H Sotiris J Ebrahim M Chua EYD Mendez JH Eng ET Landick R Götte M Chait BT Campbell EA Darst SA Nature Nature 7949 614 781 787 2023 English 10.1038/s41586-022-05664-3 36725929 unaligned raw movies /data/rawdata micrographs - multiframe TIFF TIFF 11657 50 UNSIGNED BYTE 5760 1.08 4092 1.08

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 21.4 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 52 e-/A^2; pixel size 1.08 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.

data/rawdata/21oct08b_g2e_00001gr_00014sq1200_v02_00002hln_00002enn.frames.tif