REL
2024-08-13
2026-05-31
2026-05-31
Cryo-EM structure of S.cerevisiae CHD1[L886G/L889G/L891G]-80N0/+1bp nucleosome complex
0000-0001-9195-2282
Jean-Paul
Armache
Pennsylvania State University
428 South Frear
University Park
PA
United States
16802
0000-0001-9195-2282
Jean-Paul
Armache
Pennsylvania State University
428 South Frear
University Park
PA
United States
16802
0000-0001-8025-4315
Gregory
D
Bowman
Johns Hopkins University
302 Jenkins Hall, 3400 N. Charles St.
Baltimore
MD
United States
21218
Nodelman IM
Folkwein HJ
Armache JP
Bowman GD
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01-GM084192
United States
8.4
10.6019/EMPIAR-12601
EMDB
molecule
EMD-49060
EMD-49061
EMD-49062
EMD-49063
EMD-49064
Nodelman IM
Folkwein HJ
Glime WS
Glime WS
Armache JP
A Competitive Regulatory Mechanism of the Chd1 Remodeler is Integral to Distorting Nucleosomal DNA
United States
10.1038/s41594-025-01556-y
Unaligned movies
/data/RawStacks
micrographs - multiframe
TIFF
TIFF
17624
40
UNSIGNED BYTE
11520
0.5252
8184
0.5252
The images were collected as movies in Super-Resolution mode at nominal magnification of 81,000x with a physical pixel size of 1.0504 Å (super resolution of 0.5252 Å), resulting in 17,624 images, each fractionated into 40 frames accumulating 50 e- per Å2 (0.0825 s per frame, 1.25 e- per Å per frame) total dose.
Optimized particles polished using "Bayesian Polishing" in Relion
/data/Particles
picked particles - single frame - processed
MRCS
MRCS
17428
40
SIGNED 16 BIT INTEGER
320
1.0504
320
1.0504
Particle positions in star files. Stacks in mrcs files.