REL
2025-02-28
2025-04-24
2025-04-24
Ensemble cryo-EM reveals conformational states of the nsp13 helicase replication-transcription complex
0000-0002-8241-3153
Seth
A
Darst
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
0000-0002-8241-3153
Seth
A
Darst
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
Chen J
Wang Q
Malone B
Llewellyn E
Pechersky Y
Maruthi K
Eng ET
Perry JK
Campbell EA
Shaw DE
Darst SA
Simons Foundation
SF349247
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
P41 GM103310
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24 GM129539
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM114450
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35 GM118130
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 AI161278
United States
5.3
10.6019/EMPIAR-12600
EMDB
molecule
EMD-24431
EMD-24430
EMD-24432
EMD-24428
EMD-24427
EMD-24429
EMD-24426
Chen J
Wang Q
Malone B
Llewellyn E
Pechersky Y
Maruthi K
Eng ET
Perry JK
Campbell EA
Shaw DE
Darst SA
Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex
Nature structural & molecular biology
Nat Struct Mol Biol
3
29
250
260
2022
English
10.1038/s41594-022-00734-6
35260847
unaligned raw movies
/data/20jun05a/rawdata
micrographs - multiframe
TIFF
TIFF
4358
50
UNSIGNED BYTE
5760
1.07
4092
1.07
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 24.7 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 64 e-/A^2; pixel size 1.1 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
data/20jun05a/rawdata/20jun05a_00010sq_v02_00002hln_00002enn.frames.tif
unaligned raw movies
/data/20jun10a/rawdata
micrographs - multiframe
TIFF
TIFF
13448
50
UNSIGNED BYTE
5760
1.07
4092
1.07
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 24.7 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 64 e-/A^2; pixel size 1.1 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
data/20jun10a/rawdata/20jun10a_00026sq_v02_00002hln_00002enn.frames.tif