REL
2025-02-27
2025-04-24
2025-04-24
Cryo electron microscopy of backtracked SARS-CoV-2 nsp13-replication-transcription complex
0000-0002-8241-3153
Seth
A
Darst
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
0000-0002-8241-3153
Seth
A
Darst
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
0000-0002-1332-128X
Elizabeth
A
Campbell
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
Malone B
Chen J
Darst SA
Campbell EA
Simons Foundation
SF349247
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
P41 GM103310
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24 GM129539
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
P41 GM109824
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
P41 GM103314
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35 GM118130
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM114450
United States
4.0
10.6019/EMPIAR-12598
EMDB
molecule
EMD-23007
EMD-23008
EMD-23009
Malone B
Chen J
Wang Q
Llewellyn E
Choi YJ
Olinares PDB
Cao X
Hernandez C
Eng ET
Chait BT
Shaw DE
Landick R
Darst SA
Campbell EA
Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex
Proceedings of the National Academy of Sciences of the United States of America
Proc Natl Acad Sci U S A
United States
19
118
2021
English
10.1073/pnas.2102516118
33883267
unaligned raw movies
/data/rawdata
micrographs - multiframe
TIFF
TIFF
10685
50
UNSIGNED BYTE
5760
1.065
4092
1.065
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 30 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 26.4 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 66 e-/A^2; pixel size 1.065 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
data/rawdata/n20aug12a_b1g3_00001gr_00003sq_v02_00002hln_00002enn.frames.tif