REL 2025-02-28 2025-04-09 2025-04-09 Cryo-EM of RNAP post-terminal complexes bound to RapA+ADP+AlF3 on negatively supercoiled DNA 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 Brewer JJ Inlow K Mooney RA Bosch B Olinares PDB Marcelino LP Chait BT Landick R Gelles J Campbell EA Darst SA 1.7 10.6019/EMPIAR-12595 EMDB molecule EMD-40943 Brewer JJ Inlow K Mooney RA Bosch B Olinares PDB Marcelino LP Chait BT Landick R Gelles J Campbell EA Darst SA RapA opens the RNA polymerase clamp to disrupt post-termination complexes and prevent cytotoxic R-loop formation Nature structural & molecular biology Nat Struct Mol Biol 2025 English 10.1038/s41594-024-01447-8 39779919 Unaligned raw movies /data micrographs - multiframe TIFF TIFF 20770 38 UNSIGNED BYTE 5760 1.076 4092 1.076
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 28.40 e-/ A^2/s, frame time 0.05 s (38 frames) with total exposure of 1.90 s, total dose of 53.95 e-/A^2, pixel size 1.076 A/px; defocus range -0.9 to -2.5 um; gain reference is 22jun18a_18113825_01_4092x5760_norm_0.mrc (see references folder).
data Unaligned raw movies /data micrographs - multiframe TIFF TIFF 20770 38 UNSIGNED BYTE 5760 0.844 4092 0.844
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 28.40 e-/ A^2/s, frame time 0.05 s (38 frames) with total exposure of 1.90 s, total dose of 53.95 e-/A^2, pixel size 0.844 A/px(detector); pixel size 0.422 A/px(image); defocus range -0.9 to -2.5 um; gain reference is 22jun18a_18113825_01_4092x5760_norm_0.mrc (see references folder).