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        <keyDates>
            <depositionDate>2025-02-10</depositionDate>
            <releaseDate>2025-07-25</releaseDate>
            <updateDate>2025-07-25</updateDate>
        </keyDates>
        <title>Raw data used to generate the structures of Arp2/3 complex bound to NPFs</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-0860-1639</authorORCID>
            <firstName>Kyle</firstName>
            <middleName>Roger</middleName>
            <lastName>Barrie</lastName>
            <organization type="academic">University of Pennsylvania</organization>
            <townOrCity>Philadelphia</townOrCity>
            <country>United States</country>
            <postOrZipCode>19104</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-3186-5229</authorORCID>
            <firstName>Roberto</firstName>
            <lastName>Dominguez</lastName>
            <organization type="academic">Department of Physiology, Perelman School of Medicine, University of Pennsylvania</organization>
            <townOrCity>Philadelphia</townOrCity>
            <country>United States</country>
            <postOrZipCode>19104</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0009-0009-4555-6145">Saks AJ</author>
            <author authorORCID="0000-0003-0860-1639">Barrie KR</author>
            <author authorORCID="0000-0002-0766-6302">Rebowski G</author>
            <author authorORCID="0000-0003-3186-5229">Dominguez R</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>GM073791 and GM152412</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">4.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-12564</entryDOI>
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        <scale>molecule</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-46910</emdbEntry>
            <emdbEntry>EMD-46913</emdbEntry>
            <emdbEntry>EMD-46924</emdbEntry>
            <emdbEntry>EMD-46925</emdbEntry>
            <emdbEntry>EMD-46926</emdbEntry>
            <emdbEntry>EMD-46934</emdbEntry>
            <emdbEntry>EMD-46992</emdbEntry>
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        <citationList>
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                <journalCitation published="false" preprint="false">
                    <author authorORCID="0009-0009-4555-6145" order="1">Saks AJ</author>
                    <author authorORCID="0000-0003-0860-1639" order="2">Barrie KR</author>
                    <author authorORCID="0000-0002-0766-6302" order="3">Rebowski G</author>
                    <author authorORCID="0000-0003-3186-5229" order="4">Dominguez R</author>
                    <title>NPF binding to Arp2 is allosterically linked to the release of ArpC5’s N-terminal tail and conformational changes in Arp2/3 complex</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc Natl Acad Sci USA</journalAbbreviation>
                    <country>United States</country>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2421557122</externalReferences>
                    <details>Raw movies used to generate the cryo-EM structures described in the paper.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Dataset 1</name>
        <directory>/data/beckman072524</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>5111</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
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            <pixelWidth>0.43</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.43</pixelHeight>
        </dimensions>
        <details>Dataset 1 raw movies used to generate the structures described in the paper. A gain reference is also included in data/beckman072524/gainref. The movies were collected in super-resolution mode with a pixel size of 0.43 Å/pix and binned by 2 during motion correction to a pixel size of 0.86 Å/pix.</details>
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        <name>Dataset 2</name>
        <directory>/data/beckman081324</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>8645</numImagesOrTiltSeries>
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            <imageHeight>8184</imageHeight>
            <pixelHeight>0.43</pixelHeight>
        </dimensions>
        <details>Dataset 2 raw movies used to generate the structures described in the paper. A gain reference is also included in data/beckman081324/gainref. The movies were collected in super-resolution mode with a pixel size of 0.43 Å/pix and binned by 2 during motion correction to a pixel size of 0.86 Å/pix.</details>
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